Characterization of LysBC17, a Lytic Endopeptidase from Bacillus cereus

dc.contributor.authorSwift, Steven M.
dc.contributor.authorEtobayeva, Irina V.
dc.contributor.authorReid, Kevin P.
dc.contributor.authorWaters, Jerel J.
dc.contributor.authorOakley, Brian B.
dc.contributor.authorDonovan, David M.
dc.contributor.authorNelson, Daniel C.
dc.date.accessioned2023-11-14T15:04:51Z
dc.date.available2023-11-14T15:04:51Z
dc.date.issued2019-09-19
dc.description.abstractBacillus cereus, a Gram-positive bacterium, is an agent of food poisoning. B. cereus is closely related to Bacillus anthracis, a deadly pathogen for humans, and Bacillus thuringenesis, an insect pathogen. Due to the growing prevalence of antibiotic resistance in bacteria, alternative antimicrobials are needed. One such alternative is peptidoglycan hydrolase enzymes, which can lyse Gram-positive bacteria when exposed externally. A bioinformatic search for bacteriolytic enzymes led to the discovery of a gene encoding an endolysin-like endopeptidase, LysBC17, which was then cloned from the genome of B. cereus strain Bc17. This gene is also present in the B. cereus ATCC 14579 genome. The gene for LysBC17 encodes a protein of 281 amino acids. Recombinant LysBC17 was expressed and purified from E. coli. Optimal lytic activity against B. cereus occurred between pH 7.0 and 8.0, and in the absence of NaCl. The LysBC17 enzyme had lytic activity against strains of B. cereus, B. anthracis, and other Bacillus species.
dc.description.urihttps://doi.org/10.3390/antibiotics8030155
dc.identifierhttps://doi.org/10.13016/dspace/ez6d-2lar
dc.identifier.citationSwift, S.M.; Etobayeva, I.V.; Reid, K.P.; Waters, J.J.; Oakley, B.B.; Donovan, D.M.; Nelson, D.C. Characterization of LysBC17, a Lytic Endopeptidase from Bacillus cereus. Antibiotics 2019, 8, 155.
dc.identifier.urihttp://hdl.handle.net/1903/31382
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtDepartment of Veterinary Medicineen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectpeptidoglycan hydrolase
dc.subjectendopeptidase
dc.subjectendolysin
dc.subjectautolysin
dc.subjectBacillus cereus
dc.subjectBacillus anthracis
dc.titleCharacterization of LysBC17, a Lytic Endopeptidase from Bacillus cereus
dc.typeArticle
local.equitableAccessSubmissionNo

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