Detection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressed antigens

dc.contributor.authorUpadhyay, Chitra
dc.contributor.authorAmmayappan, Arun
dc.contributor.authorVakharia, Vikram N
dc.date.accessioned2021-11-30T14:53:09Z
dc.date.available2021-11-30T14:53:09Z
dc.date.issued2009-10-07
dc.description.abstractAvian influenza viruses, belonging to the family Orthomyxoviridae, possess distinct combinations of hemagglutinin (H) and the neuraminidase (N) surface glycoproteins. Typing of both H and N antigens is essential for the epidemiological and surveillance studies. Therefore, it is important to find a rapid, sensitive, and specific method for their assay, and ELISA can be useful for this purpose, by using recombinant proteins. The nucleoprotein (NP) and truncated neuraminidase subtype 3 and 7 of avian influenza virus (AIV) were expressed in Saccharomyces cerevisiae and used to develop an indirect enzyme-linked immunosorbent assay for antibody detection. The developed assays were evaluated with a panel of 64 chicken serum samples. The performance of NP-ELISA was compared with the commercially available ProFlok® AIV ELISA kit. The results showed comparable agreement and sensitivity between the two tests, indicating that NP-ELISA assay can be used for screening the influenza type A antibody in AIV infected birds. The N3 and N7- ELISAs also reacted specifically to their type specific sera and did not exhibit any cross-reaction with heterologous neuraminidase subtype specific sera. The study demonstrates the expression of the NP, N3, and N7 proteins of AIV in yeast (S. cerevisiae) and their application in developing an indirect ELISA for detecting NP, N3 and N7 antibodies from AIV-infected chicken sera. The described indirect ELISAs are rapid, sensitive, specific and can be used as promising tests during serological surveillance.en_US
dc.description.urihttps://doi.org/10.1186/1743-422X-6-158
dc.identifierhttps://doi.org/10.13016/qgkd-ldxl
dc.identifier.citationUpadhyay, C., Ammayappan, A. & Vakharia, V.N. Detection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressed antigens. Virol J 6, 158 (2009).en_US
dc.identifier.urihttp://hdl.handle.net/1903/28166
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtDepartment of Veterinary Medicineen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectAvian Influenza Virusen_US
dc.subjectHemagglutination Inhibitionen_US
dc.subjectNewcastle Diseaseen_US
dc.subjectIndirect ELISAen_US
dc.subjectNegative Seronen_US
dc.titleDetection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressed antigensen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
1743-422X-6-158.pdf
Size:
635.95 KB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.57 KB
Format:
Item-specific license agreed upon to submission
Description: