Sustaining Interferon Induction by a High-Passage Atypical Porcine Reproductive and Respiratory Syndrome Virus Strain

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dc.contributor.authorMa, Zexu
dc.contributor.authorYu, Ying
dc.contributor.authorXiao, Yueqiang
dc.contributor.authorOpriessnig, Tanja
dc.contributor.authorWang, Rong
dc.contributor.authorYang, Liping
dc.contributor.authorNan, Yuchen
dc.contributor.authorSamal, Siba K.
dc.contributor.authorHalbur, Patrick G.
dc.contributor.authorZhang, Yan-Jin
dc.date.accessioned2017-08-31T19:52:43Z
dc.date.available2017-08-31T19:52:43Z
dc.date.issued2016-11-02
dc.descriptionPartial funding for Open Access provided by the UMD Libraries' Open Access Publishing Fund.en_US
dc.description.abstractPorcine reproductive and respiratory syndrome virus (PRRSV) strain A2MC2 induces type I interferons in cultured cells. The objective of this study was to attenuate this strain by serial passaging in MARC-145 cells and assess its virulence and immunogenicity in pigs. The A2MC2 serially passaged 90 times (A2MC2-P90) retains the feature of interferon induction. The A2MC2-P90 replicates faster with a higher virus yield than wild type A2MC2 virus. Infection of primary pulmonary alveolar macrophages (PAMs) also induces interferons. Sequence analysis showed that the A2MC2-P90 has genomic nucleic acid identity of 99.8% to the wild type but has a deletion of 543 nucleotides in nsp2. The deletion occurred in passage 60. The A2MC2-P90 genome has a total of 35 nucleotide variations from the wild type, leading to 26 amino acid differences. Inoculation of three-week-old piglets showed that A2MC2-P90 is avirulent and elicits immune response. Compared with Ingelvac PRRSĀ® MLV strain, A2MC2-P90 elicits higher virus neutralizing antibodies. The attenuated IFN inducing A2MC2-P90 should be useful for development of an improved PRRSV vaccine.en_US
dc.identifierhttps://doi.org/10.13016/M2707WP1P
dc.identifier.citationScientific Reports, 6:36312, DOI: 10.1038/srep36312en_US
dc.identifier.urihttp://hdl.handle.net/1903/19704
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtDepartment of Veterinary Medicineen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.titleSustaining Interferon Induction by a High-Passage Atypical Porcine Reproductive and Respiratory Syndrome Virus Strainen_US
dc.typeArticleen_US

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