Anaphylatoxin signaling activates macrophages to control intracellular Rickettsia proliferation

dc.contributor.authorDahmani, Mustapha
dc.contributor.authorZhu, Jinyi C.
dc.contributor.authorCook, Jack H.
dc.contributor.authorRiley, Sean P.
dc.date.accessioned2024-06-06T14:56:00Z
dc.date.available2024-06-06T14:56:00Z
dc.date.issued2023-10
dc.descriptionPartial funding for Open Access provided by the UMD Libraries' Open Access Publishing Fund.
dc.description.abstractPathogenic Rickettsia species proliferate within the cytoplasm of permissive host cells in vivo. The cytoplasm of these host cells is adequate to support the complex metabolic and physiological needs for Rickettsia growth. However, a dramatic host/pathogen interplay occurs when Rickettsia encounter innate immune cells, whereby the bacteria can proliferate as normal or the host can restrict bacterial growth. This interplay is most divergent within myeloid host cells, where intra- and extracellular factors can produce either successful Rickettsia parasitism or innate immune control of bacterial proliferation. With the prior knowledge that the mammalian complement system is activated during mammalian infection, we sought to determine if extracellular complement activation and anaphylatoxin signaling can modify the fate of Rickettsia within mononuclear host cells. Results indicate that supplementation of growth media with either C3a or C5a anaphylatoxin peptides is sufficient for many myeloid cells to control the proliferation of multiple different Rickettsia species. Chemical or genetic disruption of anaphylatoxin signaling or anaphylatoxin receptors eliminates complement-induced restriction of bacterial proliferation. Finally, anaphylatoxin signaling modifies macrophage physiology by inducing inflammatory phenotypes that ultimately control the intracellular proliferation of these pathogens.
dc.description.urihttps://doi.org/10.1128/spectrum.02538-23
dc.identifierhttps://doi.org/10.13016/aipa-tome
dc.identifier.citationDahmani M, Zhu JC, Cook JH, Riley SP. 2023. Anaphylatoxin signaling activates macrophages to control intracellular Rickettsia proliferation. Microbiol Spectr 11:e02538-23.
dc.identifier.urihttp://hdl.handle.net/1903/32597
dc.language.isoen_US
dc.publisherAmerican Society for Microbiology
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtDepartment of Veterinary Medicineen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.titleAnaphylatoxin signaling activates macrophages to control intracellular Rickettsia proliferation
dc.typeArticle
local.equitableAccessSubmissionNo

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
dahmani-et-al-2023-anaphylatoxin-signaling-activates-macrophages-to-control-intracellular-rickettsia-proliferation.pdf
Size:
4.22 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.55 KB
Format:
Item-specific license agreed upon to submission
Description: