VCAM1/VLA4 interaction mediates Ly6Clow monocyte recruitment to the brain in a TNFR signaling dependent manner during fungal infection

dc.contributor.authorSun, Donglei
dc.contributor.authorZhang, Mingshun
dc.contributor.authorSun, Peng
dc.contributor.authorLiu, Gongguan
dc.contributor.authorStrickland, Ashley B.
dc.contributor.authorChen, Yanli
dc.contributor.authorFu, Yong
dc.contributor.authorYosri, Mohammed
dc.contributor.authorShi, Meiqing
dc.date.accessioned2021-07-15T15:19:24Z
dc.date.available2021-07-15T15:19:24Z
dc.date.issued2020-02-26
dc.descriptionPartial funding for Open Access provided by the UMD Libraries' Open Access Publishing Fund.en_US
dc.description.abstractMonocytes exist in two major populations, termed Ly6C^hi and Ly6C^low monocytes. Compared to Ly6C^hi monocytes, less is known about Ly6C^low monocyte recruitment and mechanisms involved in the recruitment of this subset. Furthermore, the role of Ly6C^low monocytes during infections is largely unknown. Here, using intravital microscopy, we demonstrate that Ly6C^low monocytes are predominantly recruited to the brain vasculature following intravenous infection with Cryptococcus neoformans, a fungal pathogen causing meningoencephalitis. The recruitment depends primarily on the interaction of VCAM1 expressed on the brain endothelium with VLA4 expressed on Ly6C^low monocytes. Furthermore, TNFR signaling is essential for the recruitment through enhancing VLA4 expression on Ly6C^low monocytes. Interestingly, the recruited Ly6C^low monocytes internalized C. neoformans and carried the organism while crawling on and adhering to the luminal wall of brain vasculature and migrating to the brain parenchyma. Our study reveals a substantial recruitment of Ly6C^low monocytes to the brain and highlights important properties of this subset during infection.en_US
dc.description.urihttps://doi.org/10.1371/journal.ppat.1008361
dc.identifierhttps://doi.org/10.13016/atal-v4ln
dc.identifier.citationSun D, Zhang M, Sun P, Liu G, Strickland AB, et al. (2020) VCAM1/VLA4 interaction mediates Ly6C^low monocyte recruitment to the brain in a TNFR signaling dependent manner during fungal infection. PLOS Pathogens 16(2): e1008361.en_US
dc.identifier.urihttp://hdl.handle.net/1903/27528
dc.language.isoen_USen_US
dc.publisherPLoSen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtDepartment of Veterinary Medicineen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.titleVCAM1/VLA4 interaction mediates Ly6Clow monocyte recruitment to the brain in a TNFR signaling dependent manner during fungal infectionen_US
dc.typeArticleen_US

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