IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication

dc.contributor.authorChen, Junhao
dc.contributor.authorZhang, Ruihua
dc.contributor.authorLan, Jingjing
dc.contributor.authorLin, Shaoli
dc.contributor.authorLi, Pengfei
dc.contributor.authorGao, Jiming
dc.contributor.authorWang, Yu
dc.contributor.authorXie, Zhi-Jing
dc.contributor.authorLi, Fu-Chang
dc.contributor.authorJiang, Shi-Jin
dc.date.accessioned2023-11-14T15:48:19Z
dc.date.available2023-11-14T15:48:19Z
dc.date.issued2019-10-10
dc.description.abstractAs a disease characterized by severe liver necrosis and hemorrhage, duck viral hepatitis (DVH) is mainly caused by duck hepatitis A virus (DHAV). The positive-strand RNA genome of DHAV type 1 (DHAV-1) contains an internal ribosome entry site (IRES) element within the 5′ untranslated region (UTR), structured sequence elements within the 3′ UTR, and a poly(A) tail at the 3′ terminus. In this study, we first examined that insulin-like growth factor-2 mRNA-binding protein-1 (IGF2BP1) specifically interacted with the DHAV-1 3′ UTR by RNA pull-down assay. The interaction between IGF2BP1 and DHAV-1 3′ UTR strongly enhanced IRES-mediated translation efficiency but failed to regulate DHAV-1 replication in a duck embryo epithelial (DEE) cell line. The viral propagation of DHAV-1 strongly enhanced IGF2BP1 expression level, and viral protein accumulation was identified as the key point to this increment. Collectively, our data demonstrated the positive role of IGF2BP1 in DHAV-1 viral proteins translation and provided data support for the replication mechanism of DHAV-1.
dc.description.urihttps://doi.org/10.3390/biom9100594
dc.identifierhttps://doi.org/10.13016/dspace/ur7w-cyeg
dc.identifier.citationChen, J.; Zhang, R.; Lan, J.; Lin, S.; Li, P.; Gao, J.; Wang, Y.; Xie, Z.-J.; Li, F.-C.; Jiang, S.-J. IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication. Biomolecules 2019, 9, 594.
dc.identifier.urihttp://hdl.handle.net/1903/31390
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtDepartment of Veterinary Medicineen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectduck hepatitis A virus type 1
dc.subjectinsulin-like growth factor-2 mRNA-binding protein-1
dc.subject3' untranslated region
dc.subjecttranslation efficiency
dc.subjectviral propagation
dc.titleIGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication
dc.typeArticle
local.equitableAccessSubmissionNo

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
biomolecules-09-00594.pdf
Size:
1.88 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.55 KB
Format:
Item-specific license agreed upon to submission
Description: