IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication
dc.contributor.author | Chen, Junhao | |
dc.contributor.author | Zhang, Ruihua | |
dc.contributor.author | Lan, Jingjing | |
dc.contributor.author | Lin, Shaoli | |
dc.contributor.author | Li, Pengfei | |
dc.contributor.author | Gao, Jiming | |
dc.contributor.author | Wang, Yu | |
dc.contributor.author | Xie, Zhi-Jing | |
dc.contributor.author | Li, Fu-Chang | |
dc.contributor.author | Jiang, Shi-Jin | |
dc.date.accessioned | 2023-11-14T15:48:19Z | |
dc.date.available | 2023-11-14T15:48:19Z | |
dc.date.issued | 2019-10-10 | |
dc.description.abstract | As a disease characterized by severe liver necrosis and hemorrhage, duck viral hepatitis (DVH) is mainly caused by duck hepatitis A virus (DHAV). The positive-strand RNA genome of DHAV type 1 (DHAV-1) contains an internal ribosome entry site (IRES) element within the 5′ untranslated region (UTR), structured sequence elements within the 3′ UTR, and a poly(A) tail at the 3′ terminus. In this study, we first examined that insulin-like growth factor-2 mRNA-binding protein-1 (IGF2BP1) specifically interacted with the DHAV-1 3′ UTR by RNA pull-down assay. The interaction between IGF2BP1 and DHAV-1 3′ UTR strongly enhanced IRES-mediated translation efficiency but failed to regulate DHAV-1 replication in a duck embryo epithelial (DEE) cell line. The viral propagation of DHAV-1 strongly enhanced IGF2BP1 expression level, and viral protein accumulation was identified as the key point to this increment. Collectively, our data demonstrated the positive role of IGF2BP1 in DHAV-1 viral proteins translation and provided data support for the replication mechanism of DHAV-1. | |
dc.description.uri | https://doi.org/10.3390/biom9100594 | |
dc.identifier | https://doi.org/10.13016/dspace/ur7w-cyeg | |
dc.identifier.citation | Chen, J.; Zhang, R.; Lan, J.; Lin, S.; Li, P.; Gao, J.; Wang, Y.; Xie, Z.-J.; Li, F.-C.; Jiang, S.-J. IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication. Biomolecules 2019, 9, 594. | |
dc.identifier.uri | http://hdl.handle.net/1903/31390 | |
dc.language.iso | en_US | |
dc.publisher | MDPI | |
dc.relation.isAvailableAt | College of Agriculture & Natural Resources | en_us |
dc.relation.isAvailableAt | Department of Veterinary Medicine | en_us |
dc.relation.isAvailableAt | Digital Repository at the University of Maryland | en_us |
dc.relation.isAvailableAt | University of Maryland (College Park, MD) | en_us |
dc.subject | duck hepatitis A virus type 1 | |
dc.subject | insulin-like growth factor-2 mRNA-binding protein-1 | |
dc.subject | 3' untranslated region | |
dc.subject | translation efficiency | |
dc.subject | viral propagation | |
dc.title | IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication | |
dc.type | Article | |
local.equitableAccessSubmission | No |