Implications of the TCA Cycle on Escherichia coli Growth and Phage Replication

dc.contributor.advisorO'Hara, Jessica
dc.contributor.authorSuriaprakash, Aiswarya
dc.contributor.authorBokil, Eesha
dc.contributor.authorKoudouovoh, Carlos
dc.contributor.authorChuck, Marissa
dc.date.accessioned2021-05-07T23:10:57Z
dc.date.available2021-05-07T23:10:57Z
dc.date.issued2020
dc.description.abstractATP provides energy for the cellular processes in E. coli. The E. coli icd gene encodes the enzyme isocitrate dehydrogenase which is used in the tricarboxylic acid (TCA) cycle to make ATP. We hypothesize that T4 phage is dependent on ATP production in the host cell in order to successfully replicate. Our research aims to find if an E. coli bacteria strain lacking the icd gene will show decreased T4 phage replication because of its decreased ATP production compared to E. coli bacteria strains that possess the icd gene with normal ATP production. Decreased ATP production in the E. coli host will result in a decrease in bacterial growth rate and T4 phage replication. Plaque assays, growth curves, and lysis curves were used to monitor bacterial growth and phage replication. Host cells lacking the icd gene had decreased bacterial growth. T4 phage replication is slower in the host cells lacking the icd gene. It is the hope that these results and future research will contribute to phage therapy, a promising development in treating bacterial diseases.en_US
dc.description.sponsorshipFirst-Year Innovation and Research Experience (FIRE) at the University of Maryland, College Parken_US
dc.identifierhttps://doi.org/10.13016/tm5a-wdth
dc.identifier.urihttp://hdl.handle.net/1903/27029
dc.language.isoen_USen_US
dc.relation.isAvailableAtMaryland Center for Undergraduate Research
dc.relation.isAvailableAtDigital Repository at the University of Maryland
dc.relation.isAvailableAtUniversity of Maryland (College Park, Md)
dc.subjectHost-Pathogen Interactionsen_US
dc.subjectFIREen_US
dc.subjectSuriaprakashen_US
dc.subjectBokilen_US
dc.subjectKoudouovohen_US
dc.subjectChucken_US
dc.subjectbacteriophage replicationen_US
dc.subjecthost cell metabolism inhibitionen_US
dc.subjecticd geneen_US
dc.titleImplications of the TCA Cycle on Escherichia coli Growth and Phage Replicationen_US
dc.typePresentationen_US

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