Zika Virus Induces Degradation of the Numb Protein Required through Embryonic Neurogenesis
dc.contributor.author | He, Jia | |
dc.contributor.author | Yang, Liping | |
dc.contributor.author | Chang, Peixi | |
dc.contributor.author | Yang, Shixing | |
dc.contributor.author | Wang, Yu | |
dc.contributor.author | Lin, Shaoli | |
dc.contributor.author | Tang, Qiyi | |
dc.contributor.author | Zhang, Yanjin | |
dc.date.accessioned | 2024-06-06T14:55:48Z | |
dc.date.available | 2024-06-06T14:55:48Z | |
dc.date.issued | 2023-05-27 | |
dc.description | Partial funding for Open Access provided by the UMD Libraries' Open Access Publishing Fund. | |
dc.description.abstract | Zika virus (ZIKV) is a mosquito-borne flavivirus and causes an infection associated with congenital Zika syndrome and Guillain–Barre syndrome. The mechanism of ZIKV-mediated neuropathogenesis is not well understood. In this study, we discovered that ZIKV induces degradation of the Numb protein, which plays a crucial role in neurogenesis by allowing asymmetric cell division during embryonic development. Our data show that ZIKV reduced the Numb protein level in a time- and dose-dependent manner. However, ZIKV infection appears to have minimal effect on the Numb transcript. Treatment of ZIKV-infected cells with a proteasome inhibitor restores the Numb protein level, which suggests the involvement of the ubiquitin–proteasome pathway. In addition, ZIKV infection shortens the half-life of the Numb protein. Among the ZIKV proteins, the capsid protein significantly reduces the Numb protein level. Immunoprecipitation of the Numb protein co-precipitates the capsid protein, indicating the interaction between these two proteins. These results provide insights into the ZIKV–cell interaction that might contribute to its impact on neurogenesis. | |
dc.description.uri | https://doi.org/10.3390/v15061258 | |
dc.identifier | https://doi.org/10.13016/8upu-zlze | |
dc.identifier.citation | He, J.; Yang, L.; Chang, P.; Yang, S.; Wang, Y.; Lin, S.; Tang, Q.; Zhang, Y. Zika Virus Induces Degradation of the Numb Protein Required through Embryonic Neurogenesis. Viruses 2023, 15, 1258. | |
dc.identifier.uri | http://hdl.handle.net/1903/32596 | |
dc.language.iso | en_US | |
dc.publisher | MDPI | |
dc.relation.isAvailableAt | College of Agriculture & Natural Resources | en_us |
dc.relation.isAvailableAt | Department of Veterinary Medicine | en_us |
dc.relation.isAvailableAt | Digital Repository at the University of Maryland | en_us |
dc.relation.isAvailableAt | University of Maryland (College Park, MD) | en_us |
dc.subject | Zika virus | |
dc.subject | ZIKV | |
dc.subject | the Numb protein | |
dc.subject | the capsid protein | |
dc.title | Zika Virus Induces Degradation of the Numb Protein Required through Embryonic Neurogenesis | |
dc.type | Article | |
local.equitableAccessSubmission | No |