Zika Virus Induces Degradation of the Numb Protein Required through Embryonic Neurogenesis

dc.contributor.authorHe, Jia
dc.contributor.authorYang, Liping
dc.contributor.authorChang, Peixi
dc.contributor.authorYang, Shixing
dc.contributor.authorWang, Yu
dc.contributor.authorLin, Shaoli
dc.contributor.authorTang, Qiyi
dc.contributor.authorZhang, Yanjin
dc.date.accessioned2024-06-06T14:55:48Z
dc.date.available2024-06-06T14:55:48Z
dc.date.issued2023-05-27
dc.descriptionPartial funding for Open Access provided by the UMD Libraries' Open Access Publishing Fund.
dc.description.abstractZika virus (ZIKV) is a mosquito-borne flavivirus and causes an infection associated with congenital Zika syndrome and Guillain–Barre syndrome. The mechanism of ZIKV-mediated neuropathogenesis is not well understood. In this study, we discovered that ZIKV induces degradation of the Numb protein, which plays a crucial role in neurogenesis by allowing asymmetric cell division during embryonic development. Our data show that ZIKV reduced the Numb protein level in a time- and dose-dependent manner. However, ZIKV infection appears to have minimal effect on the Numb transcript. Treatment of ZIKV-infected cells with a proteasome inhibitor restores the Numb protein level, which suggests the involvement of the ubiquitin–proteasome pathway. In addition, ZIKV infection shortens the half-life of the Numb protein. Among the ZIKV proteins, the capsid protein significantly reduces the Numb protein level. Immunoprecipitation of the Numb protein co-precipitates the capsid protein, indicating the interaction between these two proteins. These results provide insights into the ZIKV–cell interaction that might contribute to its impact on neurogenesis.
dc.description.urihttps://doi.org/10.3390/v15061258
dc.identifierhttps://doi.org/10.13016/8upu-zlze
dc.identifier.citationHe, J.; Yang, L.; Chang, P.; Yang, S.; Wang, Y.; Lin, S.; Tang, Q.; Zhang, Y. Zika Virus Induces Degradation of the Numb Protein Required through Embryonic Neurogenesis. Viruses 2023, 15, 1258.
dc.identifier.urihttp://hdl.handle.net/1903/32596
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtDepartment of Veterinary Medicineen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectZika virus
dc.subjectZIKV
dc.subjectthe Numb protein
dc.subjectthe capsid protein
dc.titleZika Virus Induces Degradation of the Numb Protein Required through Embryonic Neurogenesis
dc.typeArticle
local.equitableAccessSubmissionNo

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