Plant genome editing with TALEN and CRISPR

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dc.contributor.authorMalzahn, Aimee
dc.contributor.authorLowder, Levi
dc.contributor.authorQi, Yiping
dc.date.accessioned2021-07-21T19:31:12Z
dc.date.available2021-07-21T19:31:12Z
dc.date.issued2017-04-24
dc.description.abstractGenome editing promises giant leaps forward in advancing biotechnology, agriculture, and basic research. The process relies on the use of sequence specific nucleases (SSNs) to make DNA double stranded breaks at user defined genomic loci, which are subsequently repaired by two main DNA repair pathways: non-homologous end joining (NHEJ) and homology directed repair (HDR). NHEJ can result in frameshift mutations that often create genetic knockouts. These knockout lines are useful for functional and reverse genetic studies but also have applications in agriculture. HDR has a variety of applications as it can be used for gene replacement, gene stacking, and for creating various fusion proteins. In recent years, transcription activator-like effector nucleases and clustered regularly interspaced palindromic repeats (CRISPR) and CRISPR associated protein 9 or CRISPR from Prevotella and Francisella 1 have emerged as the preferred SSNs for research purposes. Here, we review their applications in plant research, discuss current limitations, and predict future research directions in plant genome editing.en_US
dc.description.urihttps://doi.org/10.1186/s13578-017-0148-4
dc.identifierhttps://doi.org/10.13016/e4wp-qbxe
dc.identifier.citationMalzahn, A., Lowder, L. & Qi, Y. Plant genome editing with TALEN and CRISPR. Cell Biosci 7, 21 (2017).en_US
dc.identifier.urihttp://hdl.handle.net/1903/27558
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtPlant Science & Landscape Architectureen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectPlant genome editingen_US
dc.subjectTALENen_US
dc.subjectCRISPRen_US
dc.subjectCas9en_US
dc.subjectCpf1en_US
dc.subjectNHEJen_US
dc.subjectHDRen_US
dc.titlePlant genome editing with TALEN and CRISPRen_US
dc.typeArticleen_US

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