ECOLOGY OF A FATAL BLUE CRAB VIRUS: DETECTION, RANGE, AND PREVALENCE OF CALLINECTES SAPIDUS REO-LIKE VIRUS

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dc.contributor.advisorSchott, Eric J.en_US
dc.contributor.authorFlowers, Emily Mayaen_US
dc.contributor.departmentMarine-Estuarine-Environmental Sciencesen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2015-06-25T05:42:00Z
dc.date.available2015-06-25T05:42:00Z
dc.date.issued2015en_US
dc.description.abstractCallinectes sapidus reo-like virus (RLV) is a fatal to blue crabs and consistently found within the Chesapeake Bay. Development of a sensitive and reliable RT-qPCR assay permitted a precise assessment of RLV prevalence in crabs captured from locations near and far from soft-shell crab production sites. Viral prevalence was temporally and spatially highly variable, but outbreaks of RLV appeared to be localized to an area of 1 - 2 km2. When significantly different between sites, higher prevalence was observed near soft-shell crab production. RLV prevalence was not correlated with crab characteristics, with the exception of larger mean carapace width for infected crabs. Sequences of RLV PCR products were used to compare genotypes of outbreak and non-outbreak infections. Identical genotypes were found in outbreaks from the middle Chesapeake Bay and Long Island, NY. Together, the prevalence and genetic data are consistent with RLV outbreaks being caused by focal spread of the virus through a local population.en_US
dc.identifierhttps://doi.org/10.13016/M23G8F
dc.identifier.urihttp://hdl.handle.net/1903/16456
dc.language.isoenen_US
dc.subject.pqcontrolledEcologyen_US
dc.subject.pqcontrolledVirologyen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pquncontrolledblue craben_US
dc.subject.pquncontrolledCardoreovirusen_US
dc.subject.pquncontrolledCsRVen_US
dc.subject.pquncontrolleddisease ecologyen_US
dc.subject.pquncontrolledqPCRen_US
dc.subject.pquncontrolledreovirusen_US
dc.titleECOLOGY OF A FATAL BLUE CRAB VIRUS: DETECTION, RANGE, AND PREVALENCE OF CALLINECTES SAPIDUS REO-LIKE VIRUSen_US
dc.typeThesisen_US

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