Characterization of tick organic anion transporting polypeptides (OATPs) upon bacterial and viral infections

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dc.contributor.authorTaank, Vikas
dc.contributor.authorZhou, Wenshuo
dc.contributor.authorZhuang, Xuran
dc.contributor.authorAnderson, John F.
dc.contributor.authorPal, Utpal
dc.contributor.authorSultana, Hameeda
dc.contributor.authorNeelakanta, Girish
dc.date.accessioned2021-06-15T14:51:05Z
dc.date.available2021-06-15T14:51:05Z
dc.date.issued2018-11-14
dc.description.abstractIxodes scapularis organic anion transporting polypeptides (OATPs) play important roles in tick-rickettsial pathogen interactions. In this report, we characterized the role of these conserved molecules in ticks infected with either Lyme disease agent Borrelia burgdorferi or tick-borne Langat virus (LGTV), a pathogen closely related to tick-borne encephalitis virus (TBEV). Quantitative real-time polymerase chain reaction analysis revealed no significant changes in oatps gene expression upon infection with B. burgdorferi in unfed ticks. Synchronous infection of unfed nymphal ticks with LGTV in vitro revealed no significant changes in oatps gene expression. However, expression of specific oatps was significantly downregulated upon LGTV infection of tick cells in vitro. Treatment of tick cells with OATP inhibitor significantly reduced LGTV loads, kynurenine amino transferase (kat), a gene involved in the production of tryptophan metabolite xanthurenic acid (XA), levels and expression of several oatps in tick cells. Furthermore, bioinformatics characterization of OATPs from some of the medically important vectors including ticks, mosquitoes and lice revealed the presence of several glycosylation, phosphorylation and myristoylation sites. This study provides additional evidence on the role of arthropod OATPs in vector-intracellular pathogen interactions.en_US
dc.description.urihttps://doi.org/10.1186/s13071-018-3160-6
dc.identifierhttps://doi.org/10.13016/mu8e-up8r
dc.identifier.citationTaank, V., Zhou, W., Zhuang, X. et al. Characterization of tick organic anion transporting polypeptides (OATPs) upon bacterial and viral infections. Parasites Vectors 11, 593 (2018).en_US
dc.identifier.urihttp://hdl.handle.net/1903/27168
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtDepartment of Veterinary Medicineen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectOrganic anion transporting polypeptideen_US
dc.subjectKynurenine aminotransferaseen_US
dc.subjectBorrelia burgdorferien_US
dc.subjectLangat virusen_US
dc.subjectIxodes scapularisen_US
dc.subjectAnaplasma phagocytophilumen_US
dc.subjectXanthurenic aciden_US
dc.subjectAnti-vector vaccineen_US
dc.titleCharacterization of tick organic anion transporting polypeptides (OATPs) upon bacterial and viral infectionsen_US
dc.typeArticleen_US

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