College of Agriculture & Natural Resources
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The collections in this community comprise faculty research works, as well as graduate theses and dissertations.
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Item Sperm Quality Characterization of Male Mummichog (Fundulus heteroclitus) in Response to Legacy Contaminants(2024) Malik, Sabine; Yonkos, Lance; Environmental Science and Technology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Historically contaminated rivers persist as hazards to ecosystem and human health despite remediation attempts, impacting the species found in these ecosystems. These rivers contain complex mixtures of legacy contaminants, including dioxins, polychlorinated biphenyls, and polycyclic aromatic hydrocarbons, many of which are classified as endocrine disrupting compounds. Due to this level of contamination, few fish species are pervasive in these systems, an exception being the mummichog (Fundulus heteroclitus), known for its acquired tolerance of contaminated environmental conditions. While female and offspring reproductive success have been well-documented in the literature, few studies have investigated the use of sperm quality as a tool for assessing reproductive harm from contaminant exposure. Therefore, this study aims to demonstrate the usefulness of sperm quality characterization through the use of three assays: computer-assisted sperm analysis (CASA), a bioluminescent adenosine triphosphate (ATP) assay, and a modified Comet assay. This novel method was developed through field-collection of F. heteroclitus in three historically-contaminated tidal rivers in the United States: the Passaic River, NJ, the Christina River, DE, and the Anacostia River, MD. The results of this study not only present a novel method for investigating fish health in contaminated aquatic environments, but also a comparison of differential outcomes that can occur in rivers with varied contaminants and histories of pollution.Item Biomonitoring organochlorine compounds using bald eagles (Haliaeetus leucocephalus) in Voyageur's National Park 2011-2017 and developing new biomonitoring techniques(2018) Eberius, Rachel Ann; Bowerman, William W; Environmental Science and Technology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Wildlife are used to monitor the presence and persistence of legacy organochlorine contaminants in the environment. In this study, bald eagles (Haliaeetus leucocephalus) were utilized as an indicator of exposure to organochlorine compounds at Voyageur’s National Park, Minnesota from 2011-2017. This demonstrated decreasing concentration trends and a lack of recent inputs of organochlorine compounds. However, the use of organochlorine compounds continues in other parts of the world. Therefore, a technique for using solid phase extraction to quantify organochlorine analytes in avian plasma was developed in order to facilitate international biomonitoring of these compounds. Using this method, organochlorine compounds are extracted from plasma and stored within extraction cartridges during transport from collection site to analysis site. This has important implications for international wildlife biomonitoring. If organochlorine analytes are separated from their matrix at the site of collection, sensitive or hazardous biological materials do not need to be transported or stored.Item EXPOSURE EFFECTS OF ENVIRONMENTAL GESTAGENS ON THE REPRODUCTIVE BEHAVIOR AND FITNESS OF THE FATHEAD MINNOW (PIMEPHALES PROMELAS)(2016) Frankel, Tyler Edward; Orlando, Edward F; Animal Sciences; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Historically, endocrine disrupting chemical research has focused on environmental androgens, estrogens, thyroid hormones, and their antagonists. Recent efforts have begun to examine the effects of gestagens, which include endogenous progestogens and synthetic progestins, on aquatic organisms. Gestagens have been measured in wastewater treatment plant effluent, pulp mill effluent, and runoff from agricultural fields. While studies have documented profound effects on the reproduction of fish and amphibians, the effects of gestagen exposure on reproductive behavior and male gamete quality have been relatively unexplored. As such, a series of studies were conducted in which fathead minnows (Pimephales promelas) were exposed to either a water control, EtOH control, or one of two concentrations of gestodene (GES), levonorgestrel (LNG), or progesterone (P4) using a flow-through exposure system. Egg deposition was quantified daily, and alterations of reproductive behavior were examined on days 1, 2, and 8. After 8 days, fish were examined for the presence of secondary sexual characteristics (i.e. nuptial tubercle, fin spot, and dorsal fatpad formation). Egg deposition was affected by all treatments, with complete cessation observed at higher concentrations. Reproductive behaviors were affected after just one day in response to both treatments of LNG, GES, and P4, with effects continuing through days 2 and 8. Exposure to both concentrations of LNG and GES also caused the physical masculinization of female fathead minnows. In a second set of studies using computer assisted sperm analysis, the same treatments were utilized to determine effects on sperm motility in males as a result of in vivo or in vitro exposure. LNG and GES showed no effect in either study, and P4 caused decreases in sperm motility only as a result of in vivo exposure. Together, results from this study indicate that both GES and LNG function as environmental androgens in fishes, causing masculinization of secondary sex characteristics in females and disruption of reproduction over a short period of time. As altered reproductive behavior was observed after just one day of exposure for all treatments, this study underscores that behavior is an extremely sensitive endpoint that merits increased attention in future aquatic toxicology studies.Item BALD EAGLES (HALIAEETUS LEUCOCEPHALUS) AS INDICATORS OF GREAT LAKES ECOSYSTEM HEALTH(2016) Simon, Kendall Lyn; Bowerman, William W; Environmental Science and Technology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Environmental indicators have been proposed as a means to assess ecological integrity, monitoring both chemical and biological stressors. In this study, we used nestling bald eagles as indicators to quantify direct or indirect tertiary-level contaminant exposure. The spatial and temporal trends of polychlorinated biphenyl (PCB) congeners were evaluated in nestling plasma from 1999–2014. Two hexa-chlorinated congeners, PCB-138 and 153, were detected with the highest frequency and greatest concentrations throughout Michigan. Less-chlorinated congeners such as PCB-52 and 66 however, comprised a greater percentage of total PCB concentrations in nestlings proximate to urbanized areas, such as along the shorelines of Lake Erie. Toxic equivalents were greatest in the samples collected from nestlings located on Lake Erie, followed by the other Great Lakes spatial regions. Nestling plasma samples were also used to measure concentrations of the most heavily-used group of flame retardants, brominated diphenyl ethers (BDEs), and three groups of alternative flame retardants, non-BDE Brominated Flame Retardants (NBFRS), Dechloranes, and organophosphate esters (OPs). BDE-47, 99 and 100 contributed the greatest to total BDE concentrations. Concentrations of structurally similar NBFRs found in this study and recent atmospheric studies indicate that they are largely used as replacements to previously used BDE mixtures. A variety of Dechloranes, or derivatives of Mirex and Dechlorane Plus, were measured. Although, measured at lesser concentrations, environmental behavior of these compounds may be similar to mirex and warrant future research in aquatic species. Concentrations of OPs in nestling plasma were two to three orders of magnitude greater than all other groups of flame retardants. In addition to chemical indicators, bald eagles have also been proposed as indicators to identify ecological stressors using population measures that are tied to the fitness of individuals and populations. Using mortality as a population vitality rate, vehicle collisions were found to be the main source of mortality with a greater incidence for females during white-tailed deer (Odocoileus virginianus) hunting months and spring snow-melt. Lead poisoning was the second greatest source of mortality, with sources likely due to unretrieved hunter-killed, white-tailed deer carcasses, and possibly exacerbated by density-dependent effects due to the growing population in Michigan.Item A NOVEL NON-LETHAL LAPAROSCOPIC APPROACH TO DETECT INTERSEX (TESTICULAR OOCYTES) IN LARGEMOUTH BASS (MICROPTERUS SALMOIDES) AND SMALLMOUTH BASS (MICROPTERUS DOLOMIEU)(2016) MacLeod, Alexander Hugh; YONKOS, LANCE T; Environmental Science and Technology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)The appearance of testicular oocytes (TO) in wild fish populations has received considerable attention in the scientific literature and public media. Current methods to quantify TO are lethal; instead, a non-lethal alternative was examined. Laparoscopic insertion into the genital pore allowed internal visualization of the gonad and detection of TO by collecting five testis biopsies in smallmouth bass Micropterus dolomieu and largemouth bass Micropterus salmoides. Overall, biopsies quantified similar levels of TO detection and severity to conventional transverse sectioning with less than 10% mortality. Suitability of surgical anesthetics, tricaine methanesulfonate and electronarcosis were examined in laboratory and field applications. Electronarcosis had the added benefit of rapid sex identification and immediate release of female fish with minimal trauma, representing significant benefits when sampling small or compromised populations. Laparoscopy may be useful for monitoring the prevalence and severity of TO in these fish species when lethal sampling is not a desired outcome.Item INVESTIGATING COMPOSTING AS A METHOD FOR REDUCING ESTROGENICTY IN POULTRY LITTER AND BIOSOLIDS(2015) Hammett, Kirsten; Yonkos, Lance; Environmental Science and Technology; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Biosolids and poultry litter contain the natural estrogens 17β-estradiol and estrone, which can be transported to receiving waters via runoff when these materials are used as fertilizers. Estrogens are of concern because of their ability to act as endocrine disruptors and feminize fish. In this study, In-Vessel Aerated and Turned composting was investigated for its efficacy at mitigating estrogen concerns in BS and PL. Pre- and post- composted, BS and PL samples were investigated for total estrogenicity and estrogen species concentrations. In addition, conversion of estrone to 17β-estradiol was investigated by measuring the creation of deuterated 17β-estradiol from a deuterium-labeled estrone stock within aqueous PL mixtures. Data from these studies indicates that there may be efficacy in composting BS and PL prior to land application and suggest that estrone is capable of converting to the more potent 17β-estradiol species as a result of entering microbially active environments.Item THE EFFECTS OF POLYCHLORINATED BIPHEYNLS (PCBs) ON AVIAN CARDIAC DEVELOPMENT(2012) Carro, Tiffany; Ottinger, Mary Ann; Animal Sciences; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Polychlorinated biphenyls (PCBs) are a class of synthetic organochlorines that are thermally stable, resistant to degradation, and persistent in the environment as a result of bioaccumulation and intermittent redistribution through trophic levels. These compounds were sold commercially as mixtures in the twentieth century and later banned due to their biological toxicity. There are 209 known PCB congeners, each with different toxicities and physical properties that cause a variety of adverse health effects. Moreover, the effects of PCB mixtures vary with exposure concentrations, PCB congener toxicity, and species sensitivity. However, limited information is available about the impact of PCBs on the development of the embryonic cardiovascular system. There is a major site of contamination along the upper Hudson River in New York; wildlife in that region have shown evidence of exposure to PCBs. The purpose of this research was to determine the impact of embryonic exposure to a PCB mixture and a single congener, both found in the upper Hudson River on the developing avian cardiovascular system. In study 1, tree swallow eggs (Tachycineta bicolor) were dosed with PCB 77 and incubated to hatch. Similarly, domestic chicken eggs (Gallus domesticus) were dosed with the PCB mixture at embryonic day zero and incubated to hatch in study 2. Eggs were monitored through incubation; other measures were taken at hatch along with tissue collection. Results showed that embryonic exposure to PCBs resulted in an absence of the ventricular wall compact layer and hypertrabeculation in tree swallow hatchlings in spite of no effect on survival. Embryonic exposure to a PCB mixture in domestic chickens resulted in compact layer absence as well as additional cardiomyopathies, including absence of the ventricular wall trabeculated layer, ventricular chamber dilation, abnormal heart wall and septal formations, and arrhythmias during embryonic development. In study 3, embryonic exposure to a PCB mixture was studied at Hamburger Hamilton stages 10, 16, and 20. Embryonic exposure to a PCB mixture resulted in abnormal proliferation of cardiomyocytes early in heart development. Dose-dependent mortality occurred in chicken embryos exposed to the PCB mixture. These results support other findings demonstrating PCB effects on the cardiovascular system. Further, these data showed dramatic adverse effects of the PCB mixture as well as a single congener found in the region of the upper Hudson River on the developing avian cardiac system.