Library Faculty/Staff Scholarship and Research
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Item Lipid composition of bacterial and stable L forms of Escherichia coli W 1655 F+(1980) Baykousheva, S; Ivanova, E; Gumpert, J; Toshkov, AItem Specificities in the phospholipid and fatty acid composition of the stable protoplast type L-form of Escherichia coli B(1984) Gumpert, J.; Schade, W.; Krebs, B.; Baykousheva, S.; Ivanova, E.; Toshkov, A.The stable protoplast type L-form cells of E. coli B are twice richer in the extractable lipids than in the parent form. No qualitative differences in the phospholipid and fatty acid composition were found. However, the quantity of C14:0, C17:Δ, and C18:0 differ considerably. Unsaturated fatty acids are prevalent in the L-form cells.Item Fatty acid composition of lipids of Escherichia coli W 1655 F+ and its stable protoplast type L-form(1982) Gumpert, J.; Schade, W.; Krebs, D.; Baykousheva, S.; Ivanova, E.; Toshkov, A.The comparative fatty acid analysis of extractable and non-extractable lipids of Escherichia coli W 1655 F+ and its stable protoplast type L-form shows quantitative as well as qualitative differences. From 10 different fatty acids obtained, 16:0, 17:0 and 18:0 were present at about the same quantities in the lipid fractions of the bacterial and L-form. The absence of larger amounts of 12:0, 14:0, and 14:beta-hydroxy fatty acids in the non-extractable L-form lipids reflects the loss of the cell wall in L-form cells. The 16:1 fatty acid was found in L-form lipids, only. This qualitative difference and the 2-3 times higher content of 18:1 in L-form lipids and the 7 times lower content of cyc-19:0 in extractable lipids of the L-form may be interpreted as alterations characteristic of the changed composition of the cytoplasmic membrane in the L-form cells.