Library Faculty/Staff Scholarship and Research
Permanent URI for this collectionhttp://hdl.handle.net/1903/11
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Item Electron-microscopic demonstration of the localization of adenosinetriphosphatase in Bacillus subtilis(1984) Cherepova, N.; Baykousheva, S.; Ilieva, K.Histochemical studies and electron microscopy of Bacillus subtilis revealed the presence of ATPase in various subcellular fractions. The enzyme was preferentially localized in mesosomes, cytoplasmic membrane, periplasmic space, and cell wall.Item Suppression of serine protease secretion and sporulation in Bacillus subtilis by detergents(1987) Baykousheva, S.; Ilieva, K.In Bacillus subtilis grown under aeration at 37° for 24 h in liquid nutrient medium containing the nonionic detergents octylphenol-polyethylene glycol ether or polyoxyethylene monocetyl ether, increasing the concentrations of the detergents led to a decrease in the amount of protease secreted from the cells and abolished sporulation. Possible mechanisms by which these detergents caused these actions are discussed.Item Secretion of proteins by Bacillus subtilis 168 grown in the presence of membrane active agents (alcohols)(1988) Baykousheva, S.Sodium dodecyl sulphate-polyacrylamide gel electrophoresis was used to study the composition of proteins secreted by the Gram-positive microorganism Bacillus subtilis strain 168 when the latter was grown in the presence of primary alcohols (methanol, ethanol, 1-propanol and 1-butanol). These membrane-active agents had different effects on the pattern of proteins exported by B. subtilis. The secretion of some proteins was inhibited by the alkanols while that of others was stimulated, depending on the type of drug used. All alcohols were found to decrease the activity of extracellular enzymes such as alpha-amylase and serine protease without affecting significantly the activity of these enzymes when tested in vitro. The observed effects might be due to the ability of these agents to perturb the structure of biological membranes, thus interfering with the process of protein translocation through the lipid bilayers.