CHARACTERIZATION OF NON-CODING RNAS VIA NMR SPECTROSCOPY: ANALYSIS OF STRUCTURE, THERMAL STABILITY, AND DYNAMICS

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dc.contributor.advisorDayie, Theodore Ken_US
dc.contributor.authorNam, Hyeyeonen_US
dc.contributor.departmentBiochemistryen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2020-02-06T06:31:53Z
dc.date.available2020-02-06T06:31:53Z
dc.date.issued2019en_US
dc.description.abstractNon-coding RNAs are involved in various cellular processes and characterization of these RNAs may provide better insights into their functional roles. NMR spectroscopy is a powerful biophysical tool that can provide residue-specific information. Herein we examine an RNA triple helix at the 3' end of the lncRNA MALAT1, which may be a potential therapeutic target for cancer treatment. We investigate the local stability of the MALAT1 triple helix by analyzing the individual base-pair stability via NMR spectroscopy. In addition, we screened small molecules to identify the compounds that can selectively target the MALAT1 triple helix. In the second part, we studied the effect of dipolar couplings on the relaxation measurements of various non-coding RNAs using both computational and experimental measurements. The results suggest an increasing contribution of the dipolar coupling effect with the increasing size of the RNA.en_US
dc.identifierhttps://doi.org/10.13016/7ett-pvba
dc.identifier.urihttp://hdl.handle.net/1903/25503
dc.language.isoenen_US
dc.subject.pqcontrolledBiochemistryen_US
dc.titleCHARACTERIZATION OF NON-CODING RNAS VIA NMR SPECTROSCOPY: ANALYSIS OF STRUCTURE, THERMAL STABILITY, AND DYNAMICSen_US
dc.typeDissertationen_US

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