Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion

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dc.contributor.authorLin, Chien-Yuan
dc.contributor.authorJakes, Joseph E.
dc.contributor.authorDonohoe, Bryon S.
dc.contributor.authorCiesielski, Peter N.
dc.contributor.authorYang, Haibing
dc.contributor.authorGleber, Sophie-Charlotte
dc.contributor.authorVogt, Stefan
dc.contributor.authorDing, Shi-You
dc.contributor.authorPeer, Wendy A.
dc.contributor.authorMurphy, Angus S.
dc.contributor.authorMcCann, Maureen C.
dc.contributor.authorHimmel, Michael E.
dc.contributor.authorTucker, Melvin P.
dc.contributor.authorWei, Hui
dc.date.accessioned2021-07-26T14:36:04Z
dc.date.available2021-07-26T14:36:04Z
dc.date.issued2016-10-21
dc.description.abstractPlant lignocellulosic biomass is an abundant, renewable feedstock for the production of biobased fuels and chemicals. Previously, we showed that iron can act as a co-catalyst to improve the deconstruction of lignocellulosic biomass. However, directly adding iron catalysts into biomass prior to pretreatment is diffusion limited, and increases the cost of biorefinery operations. Recently, we developed a new strategy for expressing iron-storage protein ferritin intracellularly to accumulate iron as a catalyst for the downstream deconstruction of lignocellulosic biomass. In this study, we extend this approach by fusing the heterologous ferritin gene with a signal peptide for secretion into Arabidopsis cell walls (referred to here as FerEX). The transgenic Arabidopsis plants. FerEX. accumulated iron under both normal and iron-fertilized growth conditions; under the latter (iron-fertilized) condition, FerEX transgenic plants showed an increase in plant height and dry weight by 12 and 18 %, respectively, compared with the empty vector control plants. The SDS- and native-PAGE separation of cell-wall protein extracts followed by Western blot analyses confirmed the extracellular expression of ferritin in FerEX plants. Meanwhile, Perls' Prussian blue staining and X-ray fluorescence microscopy (XFM) maps revealed iron depositions in both the secondary and compound middle lamellae cell-wall layers, as well as in some of the corner compound middle lamella in FerEX. Remarkably, their harvested biomasses showed enhanced pretreatability and digestibility, releasing, respectively, 21 % more glucose and 34 % more xylose than the empty vector control plants. These values are significantly higher than those of our recently obtained ferritin intracellularly expressed plants. This study demonstrated that extracellular expression of ferritin in Arabidopsis can produce plants with increased growth and iron accumulation, and reduced thermal and enzymatic recalcitrance. The results are attributed to the intimate colocation of the iron co-catalyst and the cellulose and hemicellulose within the plant cell-wall region, supporting the genetic modification strategy for incorporating conversion catalysts into energy crops prior to harvesting or processing at the biorefinery.en_US
dc.description.urihttps://doi.org/10.1186/s13068-016-0639-2
dc.identifierhttps://doi.org/10.13016/yu82-jezg
dc.identifier.citationLin, CY., Jakes, J.E., Donohoe, B.S. et al. Directed plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversion. Biotechnol Biofuels 9, 225 (2016).en_US
dc.identifier.urihttp://hdl.handle.net/1903/27574
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtEnvironmental Science & Technologyen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectFerritinen_US
dc.subjectIron co-catalysten_US
dc.subjectIron accumulationen_US
dc.subjectTransgenic Arabidopsisen_US
dc.subjectBiomassen_US
dc.subjectHigh-throughput hot-water pretreatmenten_US
dc.subjectSaccharificationen_US
dc.subjectSugar releaseen_US
dc.subjectPerls’ Prussian blue stainingen_US
dc.subjectX-ray fluorescence microscopyen_US
dc.titleDirected plant cell-wall accumulation of iron: embedding co-catalyst for efficient biomass conversionen_US
dc.typeArticleen_US

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