CONSERVED ROLE OF EMX2 IN ESTABLISHING POLARITY OF SENSORY HAIR CELLS

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dc.contributor.advisorCarr, Catherine Een_US
dc.contributor.advisorWu, Doris Ken_US
dc.contributor.authorJiang, Taoen_US
dc.contributor.departmentNeuroscience and Cognitive Scienceen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2017-01-24T06:35:53Z
dc.date.available2017-01-24T06:35:53Z
dc.date.issued2016en_US
dc.description.abstractSensory hair cells in the inner ear are responsible for relaying information such as sounds and head positions to the brain. Stereocilia, which are specialized microvilli, are arranged in a staircase-pattern with the longest row sitting adjacent to the kinocilium. These two structures together form the stereociliary bundle (hair bundle), which are polarized asymmetrically at the apical surface of the hair cell. Deflection of the stereocilia towards the kinocilium opens the mechanotransduction channels at the tip of the stereocilia, which enables ion influx to depolarize the hair cell and activates action potentials in the postsynaptic neurons. Deflection towards the opposite direction results in hyperpolarization. Thus, the stereociliary bundle polarity defines the directional sensitivity of a given hair cell. Each sensory hair cell organ displays a specific pattern of stereocilia polarity. In the maculae, which detect linear acceleration in all directions, HCs can be divided into two regions with opposite polarity by a line of polarity reversal (LPR). Similar LPR is also present in the neuromast of the zebrafish lateral line system that detect pressure change of surrounding water. My results show that the homeodomain transcription factor Emx2 is essential for establishing the LPR. Expression of Emx2 in the maculae and neuromasts determines the stereocilia polarity pattern in a cell-autonomous fashion. Gain- and loss-of-function in the sensory hair cell organs of mouse and zebrafish indicate that the role of Emx2 in polarity reversal is both necessary and sufficient. In addition, my results demonstrated that Emx2 mediates this polarity reversal via one of the heterotrimer G-proteins, Gαi. In summary, my results show that Emx2 has a conserved role in dictating stereociliary bundle polarity.en_US
dc.identifierhttps://doi.org/10.13016/M29539
dc.identifier.urihttp://hdl.handle.net/1903/18957
dc.language.isoenen_US
dc.subject.pqcontrolledDevelopmental biologyen_US
dc.subject.pqcontrolledNeurosciencesen_US
dc.subject.pqcontrolledCellular biologyen_US
dc.subject.pquncontrolledCell polarityen_US
dc.subject.pquncontrolledEmx2en_US
dc.subject.pquncontrolledInner earen_US
dc.subject.pquncontrolledNeuromasten_US
dc.subject.pquncontrolledSensory hair cellen_US
dc.subject.pquncontrolledthe LPRen_US
dc.titleCONSERVED ROLE OF EMX2 IN ESTABLISHING POLARITY OF SENSORY HAIR CELLSen_US
dc.typeDissertationen_US

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