Characterization of programmed -1 ribosomal frameshift signals in the interleukin 2 receptor gamma mRNA

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dc.contributor.advisorDinman, Jonathan Den_US
dc.contributor.authorFlickinger, Zachary Flickingeren_US
dc.contributor.departmentBiologyen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2017-06-22T05:32:29Z
dc.date.available2017-06-22T05:32:29Z
dc.date.issued2016en_US
dc.description.abstractProgrammed -1 ribosomal frameshift (-1 PRF) signals are predicted to be present in ~10% of eukaryotic mRNA, suggesting a conserved role for -1 PRF in eukaryotic mRNA translation. This work focuses on -1 PRF in the interleukin 2 receptor gamma (IL2RG) mRNA. IL2RG is a component of receptors for six cytokines responsible for lymphocyte proliferation and differentiation. Altered expression of IL2RG is linked to immunodeficiency and lymphoma. We verified that the IL2RG mRNA has one potential -1 PRF signal in exon 3 that stimulates -1 frameshifting and a second in exon 8 that is definitively a -1 PRF signal. Both of these signals redirect the ribosome to a premature termination codon, suggesting -1 PRF alters IL2RG expression via NMD. Testing the effect of cancer patient associated mutations discovered in the exon 8 -1 PRF signal may elucidate a role for IL2RG -1 PRF in normal physiology and pathological phenotypes.en_US
dc.identifierhttps://doi.org/10.13016/M2HZ9C
dc.identifier.urihttp://hdl.handle.net/1903/19271
dc.language.isoenen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pquncontrolled-1 PRFen_US
dc.subject.pquncontrolledIL2RGen_US
dc.subject.pquncontrolledtranslationen_US
dc.titleCharacterization of programmed -1 ribosomal frameshift signals in the interleukin 2 receptor gamma mRNAen_US
dc.typeThesisen_US

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