The effect of freezing rate on striped bass (Morone saxatilis) spermatozoa

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dc.contributor.advisorWoods, Lewis Cen_US
dc.contributor.authorFrankel, Tyler Edwarden_US
dc.contributor.departmentAnimal Sciencesen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2013-10-10T05:33:51Z
dc.date.available2013-10-10T05:33:51Z
dc.date.issued2013en_US
dc.description.abstractSeveral studies have attempted to determine the optimal freezing rate for the cryopreservation of striped bass spermatozoa. In this study, the effects of freezing rate (-10, -15, -20 and -40<super>o</super>C/min) on sperm quality was examined utilizing Sybr-14/Propidium Iodide staining to confirm membrane integrity, a luciferin-luciferase assay to estimate ATP concentration and a Hamilton-Thorne CEROS<sup>TM</sup> system to characterize sperm motion. Males (n=12) were sampled once a week for five weeks. Samples were extended, cryo-protected using a 7.5% (v/v) dimethyl sulfoxide solution, and frozen using a controlled-rate freezer. Samples were stored in liquid nitrogen for 49 days and re-evaluated post-thaw. Sperm cryopreserved at -40<super>o</super>C per minute resulted in: total motility (10.06%), progressive motility (7.14%), ATP concentration (0.86 pmol/million cells), and sperm viability (56.5%); which were greater (P < 0.05) than the slower rates. Results demonstrate that -40<super>o</super>C/minute was the optimal freezing rate among those tested for the cryopreservation of striped bass spermatozoa.en_US
dc.identifier.urihttp://hdl.handle.net/1903/14642
dc.subject.pqcontrolledAnimal sciencesen_US
dc.subject.pqcontrolledFisheries and aquatic sciencesen_US
dc.subject.pqcontrolledPhysiologyen_US
dc.subject.pquncontrolledATPen_US
dc.subject.pquncontrolledCASAen_US
dc.subject.pquncontrolledFreezing Rateen_US
dc.subject.pquncontrolledStriped bassen_US
dc.subject.pquncontrolledViabilityen_US
dc.titleThe effect of freezing rate on striped bass (Morone saxatilis) spermatozoaen_US
dc.typeThesisen_US

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