PROTEUS MIRABILIS SURFACE SENSING SIGNAL TRANSDUCTION PATHWAY

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dc.contributor.advisorBelas, M. Roberten_US
dc.contributor.authorVargas, Vanessa Mercee Diamanteen_US
dc.contributor.departmentMarine-Estuarine-Environmental Sciencesen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2014-02-12T06:31:37Z
dc.date.available2014-02-12T06:31:37Z
dc.date.issued2013en_US
dc.description.abstractProteus mirabilis swarmer cell differentiation is induced by physical conditions that inhibit rotation of vegetative swimmer cell flagella. The protein FliL is important however, signal transduction to induce differentiation is unknown. Defects in differentiation result from mutations in genes involved in cell-wall formation and flagellar genes regulations. I hypothesized that upon surface contact, torsional stress due to inhibition of filament rotation activates stress response, induces swarmer cell differentiation and passes this signal to the protein FliL through a second protein, UmoA. My results show that the expression of stress gene cpxP changed over swarming migration with highest levels at zones of initial inoculation and areas where migration stops. I also show that complementation of fliL defect is viscosity-, temperature- and wettability-dependent, and fliL cells show improved swarming migration compared to wild-type on 0.8% agar. The study gave insight on the role of cell wall stress and fliL in swarming.en_US
dc.identifier.urihttp://hdl.handle.net/1903/14953
dc.language.isoenen_US
dc.subject.pqcontrolledMicrobiologyen_US
dc.subject.pqcontrolledGeneticsen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pquncontrolledcell wall stressen_US
dc.subject.pquncontrolledFliLen_US
dc.subject.pquncontrolledProteus mirabilisen_US
dc.subject.pquncontrolledswarmingen_US
dc.titlePROTEUS MIRABILIS SURFACE SENSING SIGNAL TRANSDUCTION PATHWAYen_US
dc.typeThesisen_US

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