Modulation of Plasma and Milk Sphingolipids in Dairy Cows Fed High-Starch Diets

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dc.contributor.authorRico, Jorge Eduardo
dc.contributor.authorSandri, Eveline C.
dc.contributor.authorSarmiento, Anrea Celemin
dc.contributor.authorLévesque, Janie
dc.contributor.authorKenéz, Ákos
dc.contributor.authorRico, Daniel C.
dc.date.accessioned2023-10-30T16:31:46Z
dc.date.available2023-10-30T16:31:46Z
dc.date.issued2021-10-19
dc.description.abstractBovine milk is a significant source of sphingolipids, dietary compounds that can exert anti-inflammatory actions, and which can modulate the host’s microbiome. Because sphingolipid synthesis can be modified by diet, we hypothesized that dietary conditions which reduced FFA availability may result in reduced sphingolipid synthesis. Twelve ruminally cannulated cows (120 ± 52 DIM; 35.5 ± 8.9 kg of milk/d; mean ± SD) were randomly assigned to treatment in a crossover design with 21-d periods. Treatments were (1) High starch (HS), (2) Control. The HS diet contained 29% starch, 24% NDF, and 2.8% fatty acids (FA), whereas the Control diet contained 20% starch, 31% NDF, and 2.3% FA. Plasma and milk samples were obtained on d 21 of each period and sphingolipids were quantified using targeted metabolomics. Univariate and multivariate analyses of generalized log-transformed and Pareto-scaled data included ANOVA (fixed effects of treatment) and discriminant analysis. The lipidomics analysis detected 71 sphingolipids across plasma and milk fat, including sphinganines (n = 3), dihydro-ceramides (n = 8), ceramides (Cer; n = 15), sphingomyelins (SM; n = 17), and glycosylated ceramides (n = 28). Followed by Cer, SM were the most abundant sphingolipids detected in milk and plasma, with a preponderance of 16:0-, 23:0-, and 24:0-carbon sidechains. Although no effects of HS diets were observed on plasma sphingolipids, we detected consistent reductions in the concentrations of several milk Cer (e.g., 22:0- and 24:0-Cer) and SM (17:0- and 23:0-SM) in response to HS. Discriminant analysis revealed distinct metabolite separation of HS and Control groups, with several Cer and SM being distinctively predictive of dietary treatment. We conclude that HS diets can reduce the secretion of milk Cer and SM, even in the absence of changes in circulating sphingolipids.
dc.description.urihttps://doi.org/10.3390/metabo11100711
dc.identifierhttps://doi.org/10.13016/dspace/dgx7-fna7
dc.identifier.citationRico, J.E.; Sandri, E.C.; Sarmiento, A.C.; Lévesque, J.; Kenéz, Á.; Rico, D.E. Modulation of Plasma and Milk Sphingolipids in Dairy Cows Fed High-Starch Diets. Metabolites 2021, 11, 711.
dc.identifier.urihttp://hdl.handle.net/1903/31179
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtAnimal & Avian Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjecthigh-starch diet
dc.subjectmilk
dc.subjectceramide
dc.subjectsphingomyelin
dc.titleModulation of Plasma and Milk Sphingolipids in Dairy Cows Fed High-Starch Diets
dc.typeArticle
local.equitableAccessSubmissionNo

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