Characterization and Analysis of the Exogenous Application of Selected Phytohormones on C. reinhardtii Metabolism

dc.contributor.advisorKahn, Jason D.
dc.contributor.authorChang, Andrew
dc.contributor.authorChang, Maria
dc.contributor.authorFeng, Patrick
dc.contributor.authorKhural, Jasjeet
dc.contributor.authorLuo, Tana
dc.contributor.authorMcCarthy, James
dc.contributor.authorMekelburg, Cory
dc.contributor.authorNadig, Kelsey
dc.contributor.authorPerry, Christine
dc.contributor.authorThaper, Sharad
dc.contributor.authorUrbanski, Rick
dc.contributor.authorVohra, Pragun
dc.contributor.authorWeber, Christian
dc.contributor.authorWong, Justin
dc.date.accessioned2011-04-05T14:24:33Z
dc.date.available2011-04-05T14:24:33Z
dc.date.issued2011
dc.descriptionGemstone Team Genes to Fuels
dc.description2011 Recipient of the Library Award for Undergraduate Research
dc.description.abstractIntroduction of algal biofuel on an industrial scale will only be possible if production cost can be lowered, either by speeding algal growth, increasing lipid production per cell, or both. Our approach examined the application of phytohormones to algae to reach this goal. Bioinformatics and literature led us to four phytohormones: auxin, brassinolide, trans-zeatin, and trans-zeatin-riboside. These were systematically introduced to algae at a range of concentrations. Auxin, brassinolide, and trans-zeatin-riboside increased algal growth rates at concentrations of 10-8M to 10-12M. A combination of auxin with trans-zeatin-riboside yielded an increased growth rate of 11%, a specific lipid mass increase of 51%, and, most notably, a lipid concentration increase of 33%. We did not find statistical significance (n=8; p>0.05) in the above changes due to biological variance and human error. However, these findings are consistent in each trial. Therefore, we believe that a treatment of auxin at 10-11M and trans-zeatin-riboside at 10-9M yield practical benefits to biodiesel production.en_US
dc.identifier.urihttp://hdl.handle.net/1903/11351
dc.language.isoen_USen_US
dc.relation.isAvailableAtDigital Repository at the University of Maryland
dc.relation.isAvailableAtUniversity of Maryland (College Park, Md)
dc.relation.isAvailableAtGemstone Program, University of Maryland (College Park, Md.)
dc.subjectbiofuelsen_US
dc.subjectalgaeen_US
dc.subjectlipidsen_US
dc.subjectphytohormonesen_US
dc.subjectbiodieselen_US
dc.subjectauxinen_US
dc.subjecttrans-zeatin-ribosideen_US
dc.subjectC. reinhardtiien_US
dc.subjectGemstone Team Genes to Fuels
dc.titleCharacterization and Analysis of the Exogenous Application of Selected Phytohormones on C. reinhardtii Metabolismen_US
dc.typeOtheren_US

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