Residual DNA in Commercial Taq DNA polymerase as a Source of Interference with Immuno-PCR assay

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dc.contributor.advisorMilton, Donald Ken_US
dc.contributor.authorGuag, Jakeen_US
dc.contributor.departmentMaryland Institute for Applied Environmental Healthen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2013-04-09T05:34:16Z
dc.date.available2013-04-09T05:34:16Z
dc.date.issued2012en_US
dc.description.abstractPolymerase Chain Reaction (PCR) was developed for a broad range of purposes. As part of developing a very sensitive Immuno-quantitative PCR (iqPCR) assay, we attempted to reproduce two of the published papers, almost always experienced false-positive amplification. Based on personal communication from one of the authors, we suspected that impure reagents were responsible for the false-positive amplification. However, PCR can amplify a small number of DNA into enormous numbers of copies and the possibility of environmental contamination cannot be excluded. In this paper we show that our primers appeared to amplify residual DNA in the Taq DNA polymerase, and induced false-positive results. This finding is not in the published methods papers for iqPCR.en_US
dc.identifier.urihttp://hdl.handle.net/1903/13865
dc.subject.pqcontrolledBiologyen_US
dc.subject.pqcontrolledEnvironmental healthen_US
dc.titleResidual DNA in Commercial Taq DNA polymerase as a Source of Interference with Immuno-PCR assayen_US
dc.typeThesisen_US

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