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- ItemEXAMINATION OF THE ASSOCIATIONS BETWEEN SELECTED PERFLUOROALKYL SUBSTANCES AND THEIR ISOMERS WITH BODY WEIGHT IN ADOLESCENTS USING NHANES 2013-2018(2023) Snyder, Jessica A; Payne-Sturges, Devon; Maryland Institute for Applied Environmental Health; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)0ABSTRACT Title of thesis: EXAMINATION OF THE ASSOCIATIONS BETWEEN SELECTED PERFLUOROALKYL SUBSTANCES AND THEIR ISOMERS WITH BODY WEIGHT IN ADOLESCENTS USING NHANES 2013-2018 Jessica Snyder, Master of Science, 2023 Thesis Directed by: Professor Devon Payne-Sturges Maryland Institute for Applied Environmental Health Perfluoroalkyl substances (PFAS), a class of environmentally persistent chemicals, are suspected of having obesogenic properties, with studies thus far being inconclusive. Few past studies of PFAS toxicity have distinguished between isomer types of each PFAS group during analysis, however. In this thesis, data for non-smoking 14-19 year-olds from three cycles of the National Health and Nutrition Examination Survey was analyzed to identify potential associations in adolescents between bodyweight and two major families of PFAS: perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) and to additionally determine if differentiating between linear and branched isomeric groups of two major PFAS families could better elucidate any associations found. In unadjusted models, weighted linear regression of body mass index (BMI) and serum concentrations of branched, linear, and total PFAS isomer groups, all returned positive associations ranging from β (95% confidence interval) of 4.06 (3.44, 4.68) for total PFOS to 12.33 (10.39,14.28) for linear PFOA. When models were adjusted for sex, age, race/ethnic origin and income level, however, only a negative association between BMI and branched PFOS (bPFOS) was found, with β= -1.24 (-2.10, -0.39). Unadjusted weighted logistic regression models of both overweight and obese status resulted in positive associations between obesity and both branched PFOS and total PFOS with odds ratios (95% confidence intervals) of 0.45 (0.27, 0.74) and 0.86 (0.75, 0.99) respectively. After adjusting for potential confounders, the only statistically significant relationship was between branched bPFOS and obesity, with a 56% decrease in the odds of obesity for every unit increase in serum concentration of bPFOS [β =-1.06, adjusted odds ratio= 0.44 (0.26,0.76)]. There was no significant difference between the sexes in any results. The apparent protective effect that bPFOS has against obesity and elevated BMI, notable as a health outcome association in itself, also illustrates that isomeric differentiation is important in studying PFAS, as nPFOS had no statistically significant 1 association by itself while ΣPFOS, used by many studies, did. Thus, the lack of differentiation could mask which PFAS type contributes to any associations discovered.
- ItemDETERMINING THE IMPACT OF WELL MAINTENANCE, CONDITION, TYPE, AND LOCATION FACTORS ON E. COLI AND TOTAL COLIFORMS IN MARYLAND FARM PRIVATE DRINKING WATER WELLS(2023) Smith, Cameron Nicole; Rosenberg Goldstein, Rachel; Maryland Institute for Applied Environmental Health; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)Even with the establishment of the Safe Drinking Water Act in 1974, private wells are still not regulated or monitored for drinking water quality or the presence of contaminants such as total coliform bacteria and Escherichia coli. The presence of microbiological contaminants in private wells poses a public health risk. With Agricultural Agents from the University of Maryland Extension, we collected 67 water samples from Maryland farms with private wells located in seven regions and 19 counties of Maryland. We evaluated water samples for total coliforms and E. coli to understand the risk of contamination for Maryland private well owners. We also analyzed the impact of well factors, location, and climate on the presence of total coliforms and E. coli in well water by analyzing participant survey responses and climate data. Our results found that 39% (26/67) of the well water samples were positive for total coliforms and 10% (7/67) were positive for E. coli. Region (p<0.01), county (p=0.03), previously testing for pH (p<0.01), and ambient temperature (p=0.05) were significant factors impacting total coliform concentration. Region (p<0.01) and precipitation in the last 24 hours of collection (p<0.01) were the only significant factors impacting E. coli concentration. These findings emphasize the importance of well water testing for private well owners.
- ItemExamining Consumer Product Use And Phthalate Exposure Among Vulnerable Populations(2023) Boyle, Meleah; Quirós-Alcalá, Lesliam; Maryland Institute for Applied Environmental Health; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)One in 13 Americans have asthma, and higher rates have been reported among women and Black children.1 In addition, hairdressers who are exposed to chemicals through products used on themselves and their clients frequently report respiratory symptoms and conditions.2–10 Limited studies indicate that chemicals in personal care and consumer products (PCP) may impact respiratory health.11–14 The goal of this dissertation is to describe PCP use and exposures to recognized and suspected respiratory irritants (phthalates) among vulnerable populations who may experience disparate exposures. The aims are to: 1) characterize PCP use among 110 children with asthma, 2) examine associations between PCP use and asthma morbidity among 110 children with asthma, and 3) characterize concentrations and exposure determinants to phthalate metabolites in post-shift urine samples among 23 female hairdressers and 17 female office workers. In aim 1, participants were majority Black (87%), males (56%), and aged 8-11 years (66%). Adolescents (12-17 years) and females reported more frequent use of hair, face, and body products compared to children (8-11 years) and males. Participants used chemical treatments on their hair as young as 4 years and females 11-16 years used feminine wipes, spray, and regular deodorant in the genital area. For aim 2, use of aerosol products, hair products, and nail polish were positively associated with maximal symptom days [number of aerosol products (aOR: 1.36; CI: 1.17, 1.59), hairspray (aOR: 1.63; CI: 1.14, 2.33), perfume (aOR: 1.40; CI: 1.11,1.77); shampoo (aOR: 1.34; CI: 1.05,1.73), hair sheen (aOR: 1.41; CI: 1.00, 2.00), nail polish (aOR: 2.42; CI: 1.72, 3.41)] among children with asthma. For Aim 3, the geometric mean (GM) for monoethyl phthalate (MEP) was 10 times higher among hairdressers (161.4 ng/mL) than office workers (15.3 ng/mL). Hairdressers who provided chemical services had higher GM MEP concentrations than those who did not: texturizing (200.2 vs. 115.4 ng/mL), relaxing (181.6 vs. 92.1 ng/mL), bleaching (182.3 vs. 71.6 ng/mL), hair color (171.9 vs. 83.2 ng/mL), and Brazilian blowout (181.4 vs. 134.6 ng/mL). Hairdressers who provided natural services had lower GM MEP concentrations than those who did not: twists (129.1 vs. 215.8 ng/mL), sister locs/locs (86.0 vs. 241.9 ng/mL), and afros (94.7 vs. 203.9 ng/mL). While larger studies are needed, this dissertation provides new data on PCP use and phthalate exposure among Black children and hairdressers.
- ItemCHARACTERIZING BACTERIAL COMMUNITIES ACROSS DIVERSE TOBACCO PRODUCTS AND DYSBIOSIS IN THE ORAL MICROBIOME RESULTING FROM TOBACCO USE(2022) Chattopadhyay, Suhana; Sapkota, Amy R; Maryland Institute for Applied Environmental Health; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)To evaluate the adverse health effects associated with tobacco smoking, the majority of studies have largely focused on the impact of chemical constituents in tobacco products and less on the impact of microbial contaminants within these products. Similarly, the United States (U.S.) Food and Drug Administration’s (FDA) Family Smoking Prevention and Tobacco Control Act (FSPTCA) requires tobacco manufacturers and importers to test and report on chemical constituents included on their list of harmful and potentially harmful constituents (HPHCs) in tobacco products and tobacco smoke; however, this HPHC list excludes microorganisms. Nevertheless, over the past decade, an increasing number of studies have explored the bacterial microbiome of tobacco products (e.g., cigarettes, little cigars, cigarillos, hookah and smokeless tobacco) and mainstream smoke. These studies have yielded robust data on bacterial diversity and bacterial community composition within individual tobacco product types. However, to date, there are no comparative characterizations of the bacterial microbiome across diverse tobacco products. In particular, there have been no studies that have characterized the metabolically-active (live) bacterial communities across these products; bacterial communities that might be transferred to the user’s oral cavity and cause subsequent adverse health effects. Furthermore, from an oral health perspective, while smoking/using tobacco products causes significant oral dysbiosis (bacterial community disturbances), there is a lack of data on the potential transfer of bacteria from tobacco products to user’s oral cavities or transient temporal changes in the oral microbiome that might result from smoking these tobacco products. Moreover, there are limited data comparing oral microbiome differences between cigarette smokers and smokeless tobacco users. To address these knowledge gaps, my dissertation research involved a comparative analysis of the bacterial microbiome of commercially-available tobacco products and anevaluation of the impacts of tobacco use on the oral microbiome of users. My specific aims were: 1) To evaluate the compositional differences in the bacterial microbiome between conventional tobacco products; 2) To evaluate transient changes in the oral microbiome of cigarette smokers after a single use of a little cigar; and 3) To conduct a comparative characterization of the oral microbiome between cigarette users, smokeless tobacco users, and non-users over time. Overall, I found that each type of conventional tobacco product harbored a significantly different bacterial community, with mentholation and/or flavoring being a significant driver of bacterial community changes. However, across all products the majority of the metabolically-active bacterial community was identified as Pseudomonas, along with members of the phylum Firmicutes, as well as a few pathogenic species previously associated with animal/human respiratory diseases. In analyzing the oral microbiome of cigarette smokers, I found that a singleuse of a little cigar product did not cause transient changes in the oral microbiome. In analyzing longer temporal effects of smoking on the oral microbiome, I found that, even though total bacterial diversity and composition did not change over time, multiple bacterial taxa were significantly different, with regard to their relative abundance, after four months. In addition, I found that dysbiosis of the oral microbiome was dependent on the type of tobacco product used (cigarettes or smokeless tobacco), and that tobacco smokers/users’ oral microbiomes harbored a more diverse set of bacterial species when compared to that of non-users. In summary, this work provides a comparative analysis of the total and metabolically-active bacterial microbiome of tobacco products, as well as rich findings regarding the relationship between tobacco use and oral microbiome dysbiosis. These data address major research priorities of the FDA relating to furthering our understanding of the adverse health risksassociated with tobacco smoking. Specifically, my data will add to the current body of knowledge regarding the potential role of tobacco bacterial communities in the development of smoking-related diseases. My data also can be leveraged by tobacco regulatory bodies to make future evidence-based policy changes that help reduce risks associated with microorganisms in tobacco products and protect public health.
- ItemEVALUATING MALARIA AND THE IMPACT OF WATER RESOURCES DEVELOPMENT IN WESTERN ETHIOPIA THROUGH A ONE HEALTH PROSPECTIVE(2022) Mansfield, Tavis; Turner, Paul; Maryland Institute for Applied Environmental Health; Digital Repository at the University of Maryland; University of Maryland (College Park, Md.)In Ethiopia, the potential to be infected with malaria may increase this decade due to the expected increases in available breeding habitats created by the filling of the Grand Ethiopian Renaissance Dam (GERD) and its reservoir as well as internal displacement and forced migration of tens of thousands of people due to the flooding of local communities by the filling of the GERD’s reservoir and ongoing civil conflict in the Tigray Region of northern Ethiopia. A One Health framework was used in this research along with refugee migration and resettlement information and, risk evaluation tools to assess the potential health effects of the construction and filling of the GERD and its reservoir in Western Ethiopia on the burden of malaria and the human population living in the same area. This thesis shows Anopheles mosquitoes that are the primary vector of malaria are present in Western Ethiopia and present an entomological surveillance tool that can be implemented in the region. The author also considers the human population movement and illustrate the current vulnerabilities of the various groups involved.