The current study assesses how neighborhood-level LGBTQ prevalence, concentrated disadvantage, residential mobility, and racial diversity and the micro-spatial presence of LGBTQ establishments uniquely and jointly predict anti-LGBTQ hate crime. Extant research utilizes neighborhood-level explanations of crime to understand anti-LGBTQ hate crime but does not account for the influence of opportunity at the micro-place, and particularly the role of LGBTQ establishments as facilitators of anti-LGBTQ crime opportunity, for understanding where anti-LGBTQ hate crimes occur. The current study uses official hate crime data, demographic data from the US Census Bureau, and publicly available data on the location of LGBTQ-centered establishments to assess the roles of neighborhood-level and micro-spatial predictors of anti-LGBTQ hate crime in Washington, DC from 2017 to 2019. Results suggest that more anti-LGBTQ hate crimes occur in places with higher LGBTQ prevalence, more residential mobility, and more LGBTQ establishments. Residential mobility also interacts with the presence of LGBTQ establishments. Findings indicate that LGBTQ establishments are associated with more risk of hate crime in less mobile (i.e., more stable) neighborhoods.