THERMAL CYCLING DESIGN ALTERNATIVES FOR THE POLYMERASE CHAIN REACTION

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dc.contributor.advisorHerold, Keith Een_US
dc.contributor.authorLewis, Monte Allenen_US
dc.contributor.departmentMechanical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2006-02-04T06:37:48Z
dc.date.available2006-02-04T06:37:48Z
dc.date.issued2005-09-27en_US
dc.description.abstractPCR (polymerase chain reaction) is a process by which a small amount of DNA is amplified many times to yield an easily detectable amount. This process is widely used for the detection of bacterial pathogens for biodefense, in basic research, criminal identification, and disease detection in humans. The reaction mixture must be cycled repeatedly between three different temperature levels in PCR. The reaction mixture is first heated at 94 °C, cooled to 54 °C, and then heated to 72 °C. This cycle is repeated 20-40 times. The main objective of the work reported here is to evaluate alternative heating/cooling schemes for PCR with the ultimate goal of speeding up a PCR reaction. A secondary goal is to arrive at a design that is consistent with battery operation to allow for a portable PCR device. Insight is gained about interactions between the PCR reaction and the engineering system.en_US
dc.format.extent3208615 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/3061
dc.language.isoen_US
dc.subject.pqcontrolledEngineering, Mechanicalen_US
dc.subject.pqcontrolledBiology, Microbiologyen_US
dc.subject.pqcontrolledEngineering, Biomedicalen_US
dc.subject.pquncontrolledPCRen_US
dc.subject.pquncontrolledMicroheatersen_US
dc.subject.pquncontrolledMicrofluidicsen_US
dc.subject.pquncontrolledPolycarbonate;Thermal Cyclersen_US
dc.titleTHERMAL CYCLING DESIGN ALTERNATIVES FOR THE POLYMERASE CHAIN REACTIONen_US
dc.typeThesisen_US

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