Effect of cargo location on translocation of cell-penetrating peptide fusion proteins into Candida albicans cells

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dc.contributor.advisorKarlsson, Amy Jen_US
dc.contributor.authorKheradmand-hajibashi, marzyehen_US
dc.contributor.departmentChemical Engineeringen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2021-09-22T05:40:15Z
dc.date.available2021-09-22T05:40:15Z
dc.date.issued2021en_US
dc.description.abstractResistance to antifungal drugs is an increasing issue in treating disease caused by the fungal pathogen Candida albicans. As a step towards new antifungal approaches, we investigated antifungal delivery of molecules to C. albicans cells using the cell-penetrating peptide, MPG, genetically fused to the model cargo, green fluorescent protein (GFP). We varied the orientation of the fusion of GFP to MPG and evaluated translocation into the cells. We found fusing the GFP to the C-terminus of the peptide resulted in translocation into almost 5% of C. albicans cells, while fusion of GFP to the N-terminus of the peptide resulted in translocation into less than 0.3% of cells. Our results indicate that fusion of cargo to the C-terminus of MPG is preferred for intracellular delivery of cargo, but further research to improve the efficiency of translocation into the cells is required.en_US
dc.identifierhttps://doi.org/10.13016/efnx-zqcm
dc.identifier.urihttp://hdl.handle.net/1903/27978
dc.language.isoenen_US
dc.subject.pqcontrolledChemical engineeringen_US
dc.titleEffect of cargo location on translocation of cell-penetrating peptide fusion proteins into Candida albicans cellsen_US
dc.typeThesisen_US

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