INVESTIGATING A PUTATIVE NON STRUCTURAL PROTEIN OF THE BIRNAVIRUS DROSOPHILA-X VIRUS

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dc.contributor.advisorWu, Louisaen_US
dc.contributor.advisorSong, Wenxiaen_US
dc.contributor.authorSavage, Jason Ericen_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2004-08-27T05:39:52Z
dc.date.available2004-08-27T05:39:52Z
dc.date.issued2004-08-12en_US
dc.description.abstractDrosophila-X Virus (DXV) is the prototype virus of the Entomobirnavirus genera in the family Birnaviridae; its genome consists of two double-stranded RNA segments. DXV has two open reading frames on segment A, one encoding a polyprotein and the other capable of encoding a putative 27-kDa non structural protein (DXV-NS). This project investigated the existence of DXV-NS since this putative protein is unique to Birnaviridae. Research into the nature of DXV-NS was furthered by the development of a number of tools: recombinant baculovirus and a stable Drosophila cell line expressing DXV-NS; an antibody against NS; a reporter construct to test a potential -1 frameshift signal. Though in the course of this research much was learned about DXV-NS, the greater question as to whether this open reading frame is expressed by DXV or is merely an artifact remains elusive.en_US
dc.format.extent4690322 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/1841
dc.language.isoen_US
dc.subject.pqcontrolledBiology, Molecularen_US
dc.subject.pquncontrolledDrosophila-X Virusen_US
dc.subject.pquncontrolledBirnavirusen_US
dc.subject.pquncontrolledNon-structural Proteinen_US
dc.subject.pquncontrolledDXVen_US
dc.titleINVESTIGATING A PUTATIVE NON STRUCTURAL PROTEIN OF THE BIRNAVIRUS DROSOPHILA-X VIRUSen_US
dc.typeThesisen_US

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