CTL-derived exosomes enhance the activation of CTLs stimulated by low affinity peptides

dc.contributor.advisorXiao, Zhengguoen_US
dc.contributor.authorWu, Shu-Weien_US
dc.contributor.departmentAnimal Sciencesen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2018-09-12T05:37:19Z
dc.date.available2018-09-12T05:37:19Z
dc.date.issued2018en_US
dc.description.abstractCytotoxic T cell (CTL) is the key to induce an effective immune response against infections caused by intracellular pathogens, such as virus and cancer. CTLs with low affinity can induce and maintain a substantial population during an adaptive immune response, although CTLs with a highest-affinity receive competitive activation signals. Low-affinity CTLs are important to induce effector response and maintain a diverse memory repertoire. However, the mechanism of generating and maintaining the expansion of lower affinity CTLs is still unknown. Here, we showed that the presence of exosome (Exo) enhanced the CTL survival and increased the cell proliferation especially in CTLs treated with the low-affinity peptide. Exo together with peptides also improved the production of IFN-γ and GZB. The exosomal stimulation in CTLs was relative to up-regulation of CD25 expression, which enhanced the IL-2 survival signals. Moreover, Exo derived from an early stage of activation had a similar but weaker function comparing with Exo derived from a late stage of activation in activating CTLs. This study identified the important role of the exosome derived from CTLs stimulated by the highest-affinity peptide in activating the naïve T cells stimulated by the low-affinity peptide.en_US
dc.identifierhttps://doi.org/10.13016/M2BN9X631
dc.identifier.urihttp://hdl.handle.net/1903/21220
dc.language.isoenen_US
dc.subject.pqcontrolledImmunologyen_US
dc.subject.pqcontrolledBiologyen_US
dc.subject.pquncontrolledbystander activaionen_US
dc.subject.pquncontrolledCD8+ T cellsen_US
dc.subject.pquncontrolledCTLsen_US
dc.subject.pquncontrolledexosomesen_US
dc.subject.pquncontrolledlow affinity T cellsen_US
dc.subject.pquncontrolledT cell activationen_US
dc.titleCTL-derived exosomes enhance the activation of CTLs stimulated by low affinity peptidesen_US
dc.typeThesisen_US

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