Identification and Quantification of Secreted Proteins Driving Hematopoiesis in an Induced Pluripotent Stem Cell Coculture System

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dc.contributor.advisorWang, Nam Sun
dc.contributor.authorAmedeo, Michael
dc.contributor.authorChowdhary, Prableen K
dc.contributor.authorJalalian, Aria
dc.contributor.authorLai, Wei Chen
dc.contributor.authorSahai, Amil
dc.contributor.authorThomas, George
dc.contributor.authorUppalapati, Akhil
dc.contributor.authorVejzagic, Farah
dc.date.accessioned2018-06-22T17:39:18Z
dc.date.available2018-06-22T17:39:18Z
dc.date.issued2018
dc.description.abstractAcute myeloid leukemia (AML) results from a genetic alteration in hematopoietic stem cells (HSCs). Recent treatment techniques focused on the utilization of induced pluripotent stem cells (iPSC) as an avenue to introduce HSCs in patients with AML. In this study, we analyze whether OP9 cells assist in HSC differentiation through direct cell signaling or indirect cell signaling of iPSCs. We measured the expression levels of relevant cytokines known to play a role in hematopoiesis. Our data showed a significant upregulation of all the proteins we analyzed in the iPSC/OP9 direct coculture and significant downregulation in the iPSC/OP9 indirect coculture. Our results indicate that direct cell to cell contact with OP9 cells may be necessary to induce differentiation of iPSCs.en_US
dc.identifierhttps://doi.org/10.13016/M2GM81R8C
dc.identifier.urihttp://hdl.handle.net/1903/20670
dc.language.isoen_USen_US
dc.relation.isAvailableAtDigital Repository at the University of Maryland
dc.relation.isAvailableAtGemstone Program, University of Maryland (College Park, Md)
dc.subjectGemstone Team BLOODen_US
dc.titleIdentification and Quantification of Secreted Proteins Driving Hematopoiesis in an Induced Pluripotent Stem Cell Coculture Systemen_US
dc.typeThesisen_US

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