Physiological Effects of Alcohol on Crayfish Escape Circuitry

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dc.contributor.advisorHerberholz, Jensen_US
dc.contributor.authorSwierzbinski, Matthew Edwarden_US
dc.contributor.departmentNeuroscience and Cognitive Scienceen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2016-09-08T05:40:12Z
dc.date.available2016-09-08T05:40:12Z
dc.date.issued2016en_US
dc.description.abstractAlcohol is one of the oldest and most widely used drugs on the planet, but the cellular mechanisms by which it affects neural function are still poorly understood. Unlike other drugs of abuse, alcohol has no specific receptor in the nervous system, but is believed to operate through GABAergic and serotonergic neurotransmitter systems. Invertebrate models offer circuits of reduced numerical complexity and involve the same cell types and neurotransmitter systems as vertebrate circuits. The well-understood neural circuits controlling crayfish escape behavior offer neurons that are modulated by GABAergic inhibition, thus making tail-flip circuitry an effective circuit model to study the cellular mechanisms of acute alcohol exposure. Crayfish are capable of two stereotyped, reflexive escape behaviors known as tail-flips that are controlled by two different pairs of giant interneurons, the lateral giants (LG) and the medial giants (MG). The LG circuit has been an established model in the neuroscience field for more than 60 years and is almost completely mapped out. In contrast, the MG is still poorly understood, but has important behavioral implications in social behavior and value-based decision making. In this dissertation, I show that both crayfish tail-flip circuitry are physiologically sensitive to relevant alcohol concentrations and that this sensitivity is observable on the single cell level. I also show that this ethyl alcohol (EtOH) sensitivity in the LG can be changed by altering the crayfish’s recent social experience and by removing descending inputs to the LG. While the MG exhibits similar physiological sensitivity, its inhibitory properties have never been studied before this research. Through the use of electrophysiological and pharmacological techniques, I show that the MG exhibits many similar inhibitory properties as the LG that appear to be the result of GABA-mediated chloride currents. Finally, I present evidence that the EtOH-induced changes in the MG are blocked through pre-treatment of the potent GABAA receptor agonist, muscimol, which underlines the role of GABA in EtOH’s effects on crayfish tail-flip circuitry. The work presented here opens the way for crayfish tail-flip circuitry to be used as an effective model for EtOH’s acute effects on aggression and value-based decision making.en_US
dc.identifierhttps://doi.org/10.13016/M2NB9D
dc.identifier.urihttp://hdl.handle.net/1903/18761
dc.language.isoenen_US
dc.subject.pqcontrolledNeurosciencesen_US
dc.subject.pqcontrolledCellular biologyen_US
dc.subject.pqcontrolledPhysiologyen_US
dc.subject.pquncontrolledAlcoholen_US
dc.subject.pquncontrolledCrayfishen_US
dc.subject.pquncontrolledGABAen_US
dc.subject.pquncontrolledLateral Gianten_US
dc.subject.pquncontrolledMedial Gianten_US
dc.subject.pquncontrolledSocialen_US
dc.titlePhysiological Effects of Alcohol on Crayfish Escape Circuitryen_US
dc.typeDissertationen_US

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