Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo

dc.contributor.authorAsfor, Amin S.
dc.contributor.authorNazki, Salik
dc.contributor.authorReddy, Vishwanatha R.A.P.
dc.contributor.authorCampbell, Elle
dc.contributor.authorDulwich, Katherine L.
dc.contributor.authorGiotis, Efstathios S.
dc.contributor.authorSkinner, Michael A.
dc.contributor.authorBroadbent, Andrew J.
dc.date.accessioned2023-10-31T15:20:18Z
dc.date.available2023-10-31T15:20:18Z
dc.date.issued2021-05-18
dc.description.abstractIn order to better understand differences in the outcome of infectious bursal disease virus (IBDV) infection, we inoculated a very virulent (vv) strain into White Leghorn chickens of inbred line W that was previously reported to experience over 24% flock mortality, and three inbred lines (15I, C.B4 and 0) that were previously reported to display no mortality. Within each experimental group, some individuals experienced more severe disease than others but line 15I birds experienced milder disease based on average clinical scores, percentage of birds with gross pathology, average bursal lesion scores and average peak bursal virus titre. RNA-Seq analysis revealed that more severe disease in line W was associated with significant up-regulation of pathways involved in inflammation, cytoskeletal regulation by Rho GTPases, nicotinic acetylcholine receptor signaling, and Wnt signaling in the bursa compared to line 15I. Primary bursal cell populations isolated from uninfected line W birds contained a significantly greater percentage of KUL01+ macrophages than cells isolated from line 15I birds (p < 0.01) and, when stimulated ex vivo with LPS, showed more rapid up-regulation of pro-inflammatory gene expression than those from line 15I birds. We hypothesize that a more rapid induction of pro-inflammatory cytokine responses in bursal cells following IBDV infection leads to more severe disease in line W birds than in line 15I.
dc.description.urihttps://doi.org/10.3390/v13050933
dc.identifierhttps://doi.org/10.13016/dspace/ipvk-xxd2
dc.identifier.citationAsfor, A.S.; Nazki, S.; Reddy, V.R.A.P.; Campbell, E.; Dulwich, K.L.; Giotis, E.S.; Skinner, M.A.; Broadbent, A.J. Transcriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo. Viruses 2021, 13, 933.
dc.identifier.urihttp://hdl.handle.net/1903/31228
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtAnimal & Avian Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectIBDV
dc.subjectinfectious bursal disease
dc.subjectbursa of Fabricius
dc.subjectinbred lines
dc.subjectchickens
dc.titleTranscriptomic Analysis of Inbred Chicken Lines Reveals Infectious Bursal Disease Severity Is Associated with Greater Bursal Inflammation In Vivo and More Rapid Induction of Pro-Inflammatory Responses in Primary Bursal Cells Stimulated Ex Vivo
dc.typeArticle
local.equitableAccessSubmissionNo

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