Genome-wide recombination map construction from single sperm sequencing in cattle

Simple item page
dc.contributor.authorYang, Liu
dc.contributor.authorGao, Yahui
dc.contributor.authorLi, Mingxun
dc.contributor.authorPark, Ki-Eun
dc.contributor.authorLiu, Shuli
dc.contributor.authorKang, Xiaolong
dc.contributor.authorLiu, Mei
dc.contributor.authorOswalt, Adam
dc.contributor.authorFang, Lingzhao
dc.contributor.authorTelugu, Bhanu P.
dc.contributor.authorSattler, Charles G.
dc.contributor.authorLi, Cong-jun
dc.contributor.authorCole, John B.
dc.contributor.authorSeroussi, Eyal
dc.contributor.authorXu, Lingyang
dc.contributor.authorYang, Lv
dc.contributor.authorZhou, Yang
dc.contributor.authorLi, Li
dc.contributor.authorZhang, Hongping
dc.contributor.authorRosen, Benjamin D.
dc.contributor.authorVan Tassell, Curtis P.
dc.contributor.authorMa, Li
dc.contributor.authorLiu, George E.
dc.date.accessioned2022-07-25T14:24:39Z
dc.date.available2022-07-25T14:24:39Z
dc.date.issued2022-03-05
dc.description.abstractMeiotic recombination is one of the important phenomena contributing to gamete genome diversity. However, except for human and a few model organisms, it is not well studied in livestock, including cattle. To investigate their distributions in the cattle sperm genome, we sequenced 143 single sperms from two Holstein bulls. We mapped meiotic recombination events at high resolution based on phased heterozygous single nucleotide polymorphism (SNP). In the absence of evolutionary selection pressure in fertilization and survival, recombination events in sperm are enriched near distal chromosomal ends, revealing that such a pattern is intrinsic to the molecular mechanism of meiosis. Furthermore, we further validated these findings in single sperms with results derived from sequencing its family trio of diploid genomes and our previous studies of recombination in cattle. To our knowledge, this is the first large-scale single sperm whole-genome sequencing effort in livestock, which provided useful information for future studies of recombination, genome instability, and male infertility.en_US
dc.description.urihttps://doi.org/10.1186/s12864-022-08415-w
dc.identifierhttps://doi.org/10.13016/qlfi-qliy
dc.identifier.citationYang, L., Gao, Y., Li, M. et al. Genome-wide recombination map construction from single sperm sequencing in cattle. BMC Genomics 23, 181 (2022).en_US
dc.identifier.urihttp://hdl.handle.net/1903/29071
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtAnimal & Avian Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectCattleen_US
dc.subjectSingle spermen_US
dc.subjectSequencingen_US
dc.subjectRecombinationen_US
dc.titleGenome-wide recombination map construction from single sperm sequencing in cattleen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s12864-022-08415-w.pdf
Size:
2.2 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.57 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Animal & Avian Sciences Research Works