Marek’s disease virus infection induces widespread differential chromatin marks in inbred chicken lines

dc.contributor.authorMitra, Apratim
dc.contributor.authorLuo, Juan
dc.contributor.authorZhang, Huanming
dc.contributor.authorCui, Kairong
dc.contributor.authorZhao, Keji
dc.contributor.authorSong, Jiuzhou
dc.description.abstractMarek’s disease (MD) is a neoplastic disease in chickens caused by the MD virus (MDV). Successful vaccine development against MD has resulted in increased virulence of MDV and the understanding of genetic resistance to the disease is, therefore, crucial to long-term control strategies. Also, epigenetic factors are believed to be one of the major determinants of disease response. Here, we carried out comprehensive analyses of the epigenetic landscape induced by MDV, utilizing genome-wide histone H3 lysine 4 and lysine 27 trimethylation maps from chicken lines with varying resistance to MD. Differential chromatin marks were observed on genes previously implicated in the disease such as MX1 and CTLA-4 and also on genes reported in other cancers including IGF2BP1 and GAL. We detected bivalent domains on immune-related transcriptional regulators BCL6, CITED2 and EGR1, which underwent dynamic changes in both lines as a result of MDV infection. In addition, putative roles for GAL in the mechanism of MD progression were revealed. Our results confirm the presence of widespread epigenetic differences induced by MD in chicken lines with different levels of genetic resistance. A majority of observed epigenetic changes were indicative of increased levels of viral infection in the susceptible line symptomatic of lowered immunocompetence in these birds caused by early cytolytic infection. The GAL system that has known anti-proliferative effects in other cancers is also revealed to be potentially involved in MD progression. Our study provides further insight into the mechanisms of MD progression while revealing a complex landscape of epigenetic regulatory mechanisms that varies depending on host factors.en_US
dc.identifier.citationMitra, A., Luo, J., Zhang, H. et al. Marek’s disease virus infection induces widespread differential chromatin marks in inbred chicken lines. BMC Genomics 13, 557 (2012).en_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtAnimal & Avian Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectHistone modificationsen_US
dc.subjectDifferential marksen_US
dc.subjectBivalent domainen_US
dc.subjectChromatin signatureen_US
dc.subjectMarek’s diseaseen_US
dc.titleMarek’s disease virus infection induces widespread differential chromatin marks in inbred chicken linesen_US


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