An EST resource for tilapia based on 17 normalized libraries and assembly of 116,899 sequence tags

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dc.contributor.authorLee, Bo-Young
dc.contributor.authorHowe, Aimee E
dc.contributor.authorConte, Matthew A
dc.contributor.authorD'Cotta, Helena
dc.contributor.authorPepey, Elodie
dc.contributor.authorBaroiller, Jean-Francois
dc.contributor.authordi Palma, Federica
dc.contributor.authorCarleton, Karen L
dc.contributor.authorKocher, Thomas D
dc.date.accessioned2021-11-15T19:57:26Z
dc.date.available2021-11-15T19:57:26Z
dc.date.issued2010-04-30
dc.description.abstractLarge collections of expressed sequence tags (ESTs) are a fundamental resource for analysis of gene expression and annotation of genome sequences. We generated 116,899 ESTs from 17 normalized and two non-normalized cDNA libraries representing 16 tissues from tilapia, a cichlid fish widely used in aquaculture and biological research. The ESTs were assembled into 20,190 contigs and 36,028 singletons for a total of 56,218 unique sequences and a total assembled length of 35,168,415 bp. Over the whole project, a unique sequence was discovered for every 2.079 sequence reads. 17,722 (31.5%) of these unique sequences had significant BLAST hits (e-value < 10-10) to the UniProt database. Normalization of the cDNA pools with double-stranded nuclease allowed us to efficiently sequence a large collection of ESTs. These sequences are an important resource for studies of gene expression, comparative mapping and annotation of the forthcoming tilapia genome sequence.en_US
dc.description.urihttps://doi.org/10.1186/1471-2164-11-278
dc.identifierhttps://doi.org/10.13016/tfws-yw7l
dc.identifier.citationLee, BY., Howe, A.E., Conte, M.A. et al. An EST resource for tilapia based on 17 normalized libraries and assembly of 116,899 sequence tags. BMC Genomics 11, 278 (2010).en_US
dc.identifier.urihttp://hdl.handle.net/1903/28117
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Computer, Mathematical & Physical Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtBiologyen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectGene Ontologyen_US
dc.subjectCichlid Fishen_US
dc.subjectCone Opsinen_US
dc.subjectHaplochromine Cichliden_US
dc.subjectUniProt Entryen_US
dc.titleAn EST resource for tilapia based on 17 normalized libraries and assembly of 116,899 sequence tagsen_US
dc.typeArticleen_US

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