In situ Activity of NAC11-7 roseobacters in Coastal Waters off the Chesapeake Bay based on ftsZ Expression

dc.contributor.advisorSuzuki, Marcelino Ten_US
dc.contributor.authorYao, Daohongen_US
dc.contributor.departmentMarine-Estuarine-Environmental Sciencesen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.description.abstractPhylogenetic analysis of sequences of the cell division gene ftsZ retrieved from the Atlantic coast revealed an interesting subgroup NAC11-7, which was targeted by a specifically designed and optimized Taqman assay in diel samples collected in situ and in parallel on-deck incubations. Rapid changes of ftsZ gene copies and the patchy distribution of other phylotypes at different time points suggested that different NAC11-7 populations were sampled. Strong correlations between ftsZ expression and gene abundance (r-squared=0.62), and between ftsZ expression and water temperature (r-squared=0.73) for in situ samples suggested non-synchronous growth of NAC11-7 group. Contrastingly, a sharp 9:00 AM peak of ftsZ expression in the on deck incubation experiment suggested synchronous growth. We propose a possible mixed model in which a certain fraction of the population is synchronously dividing, while a background of asynchronously dividing NAC11-7 cells also exist, some of which are expressing ftsZ at any given time.en_US
dc.format.extent1138610 bytes
dc.subject.pqcontrolledBiology, Microbiologyen_US
dc.titleIn situ Activity of NAC11-7 roseobacters in Coastal Waters off the Chesapeake Bay based on ftsZ Expressionen_US
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