Volumetric super-resolution microscopy approaches for investigating synaptic connectivity in the mammalian visual system
| dc.contributor.advisor | Speer, Colenso | |
| dc.contributor.author | Vatan, Tarlan | |
| dc.date.accessioned | 2020-04-20T16:42:06Z | |
| dc.date.available | 2020-04-20T16:42:06Z | |
| dc.date.issued | 2020 | |
| dc.description.abstract | Imaging synapses in the brain is difficult due to the diffraction limit of light microscopy, which limits image resolution to ~200nm laterally and ~600nm axially. Super-resolution fluorescence microscopy techniques circumvent this problem, allowing us to visualize subsynaptic molecular interactions. Stochastic Optical Reconstruction Microscopy (STORM) is a single molecule imaging technique that relies on stochastic photoswitching of organic dyes between fluorescent and non-fluorescent to states to produce a resolution of ~20nm laterally and ~50nm axially. Together with ultra-thin serial sectioning, this approach allows for the collection of volumetric super-resolution data. Expansion Microscopy (ExM), on the other hand, is a different super-resolution approach that does not rely on special dyes or instruments. ExM achieves sub-diffraction-limit image resolution by physically expanding the specimen within a swellable polyacrylamide matrix. This technique is exciting in that it offers a simple, fast, and inexpensive method of achieving high image resolution. | en_US |
| dc.description.sponsorship | Maryland Summer Scholars Program | en_US |
| dc.identifier | https://doi.org/10.13016/tvat-6vqw | |
| dc.identifier.uri | http://hdl.handle.net/1903/25820 | |
| dc.language.iso | en_US | en_US |
| dc.relation.isAvailableAt | Maryland Center for Undergraduate Research | |
| dc.relation.isAvailableAt | Digital Repository at the University of Maryland | |
| dc.relation.isAvailableAt | University of Maryland (College Park, Md) | |
| dc.subject | Biology | en_US |
| dc.subject | CMNS | en_US |
| dc.subject | Vatan | en_US |
| dc.subject | MSS | en_US |
| dc.subject | synaptic connectivity | en_US |
| dc.subject | Super-resolution microscopy | en_US |
| dc.subject | Expansion Microscopy (ExM) | en_US |
| dc.subject | Stochastic Optical Reconstruction Microscopy (STORM) | en_US |
| dc.subject | visual system | en_US |
| dc.title | Volumetric super-resolution microscopy approaches for investigating synaptic connectivity in the mammalian visual system | en_US |
| dc.type | Presentation | en_US |