Induction of apoptosis in HeLa cells by chloroform fraction of seed extracts of Nigella sativa

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dc.contributor.authorShafi, Gowhar
dc.contributor.authorMunshi, Anjana
dc.contributor.authorHasan, Tarique N
dc.contributor.authorAlshatwi, Ali A
dc.contributor.authorJyothy, A
dc.contributor.authorLei, David KY
dc.date.accessioned2021-11-18T18:00:39Z
dc.date.available2021-11-18T18:00:39Z
dc.date.issued2009-11-27
dc.description.abstractCancer remains one of the most dreaded diseases causing an astonishingly high death rate, second only to cardiac arrest. The fact that conventional and newly emerging treatment procedures like chemotherapy, catalytic therapy, photodynamic therapy and radiotherapy have not succeeded in reverting the outcome of the disease to any drastic extent, has made researchers investigate alternative treatment options. The extensive repertoire of traditional medicinal knowledge systems from various parts of the world are being re-investigated for their healing properties. This study progresses in the direction of identifying component(s) from Nigella sativa with anti cancer acitivity. In the present study we investigated the efficacy of Organic extracts of Nigella sativa seed powder for its clonogenic inhibition and induction of apoptosis in HeLa cancer cell. Methanolic, n-Hexane and chloroform extracts of Nigella sativa seedz effectively killed HeLa cells. The IC50 values of methanolic, n-hexane, and chloroform extracts of Nigella sativa were 2.28 μg/ml, 2.20 μg/ml and 0.41 ng/ml, respectively. All three extracts induced apoptosis in HeLa cells. Apoptosis was confirmed by DNA fragmentation, western blot and terminal transferase-mediated dUTP-digoxigenin-end labeling (TUNEL) assay. Western Blot and TUNEL results suggested that Nigella sativa seed extracts regulated the expression of pro- and anti- apoptotic genes, indicating its possible development as a potential therapeutic agent for cervical cancer upon further investigation.en_US
dc.identifierhttps://doi.org/10.13016/xbge-pdau
dc.identifier.citationShafi, G., Munshi, A., Hasan, T.N. et al. Induction of apoptosis in HeLa cells by chloroform fraction of seed extracts of Nigella sativa. Cancer Cell Int 9, 29 (2009).en_US
dc.identifier.urihttp://hdl.handle.net/1903/28146
dc.identifier.urihttps://doi.org/10.1186/1475-2867-9-29
dc.language.isoen_USen_US
dc.publisherSpringer Natureen_US
dc.relation.isAvailableAtCollege of Agriculture & Natural Resourcesen_us
dc.relation.isAvailableAtNutrition & Food Scienceen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.subjectCervical Canceren_US
dc.subjectHeLa Cellen_US
dc.subjectMethanolic Extracten_US
dc.subjectChloroform Extracten_US
dc.subjectHerbal Decoctionen_US
dc.titleInduction of apoptosis in HeLa cells by chloroform fraction of seed extracts of Nigella sativaen_US
dc.typeArticleen_US

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