QUANTITY: An Isobaric Tag for Quantitative Glycomics
QUANTITY: An Isobaric Tag for Quantitative Glycomics
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Date
2015
Authors
Yang, Shuang
Wang, Meiyao
Chen, Lijun
Yin, Bojiao
Song, Guoqiang
Turko, Illarion V.
Phinney, Karen W.
Betenbaugh, Michael J.
Zhang, Hui
Li, Shuwei
Advisor
Citation
Scientific Reports (2015) 5:17585 doi: 10.1038/srep17585
DRUM DOI
Abstract
Glycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics - analysis of glycans at global level - however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering.
Notes
Funding for Open Access provided by the UMD Libraries Open Access Publishing Fund.