QUANTITY: An Isobaric Tag for Quantitative Glycomics

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dc.contributor.authorYang, Shuang
dc.contributor.authorWang, Meiyao
dc.contributor.authorChen, Lijun
dc.contributor.authorYin, Bojiao
dc.contributor.authorSong, Guoqiang
dc.contributor.authorTurko, Illarion V.
dc.contributor.authorPhinney, Karen W.
dc.contributor.authorBetenbaugh, Michael J.
dc.contributor.authorZhang, Hui
dc.contributor.authorLi, Shuwei
dc.date.accessioned2018-05-17T14:33:01Z
dc.date.available2018-05-17T14:33:01Z
dc.date.issued2015
dc.descriptionFunding for Open Access provided by the UMD Libraries Open Access Publishing Fund.en_US
dc.description.abstractGlycan is an important class of macromolecules that play numerous biological functions. Quantitative glycomics - analysis of glycans at global level - however, is far behind genomics and proteomics owing to technical challenges associated with their chemical properties and structural complexity. As a result, technologies that can facilitate global glycan analysis are highly sought after. Here, we present QUANTITY (Quaternary Amine Containing Isobaric Tag for Glycan), a quantitative approach that can not only enhance detection of glycans by mass spectrometry, but also allow high-throughput glycomic analysis from multiple biological samples. This robust tool enabled us to accomplish glycomic survey of bioengineered Chinese Hamster Ovary (CHO) cells with knock-in/out enzymes involved in protein glycosylation. Our results demonstrated QUANTITY is an invaluable technique for glycan analysis and bioengineering.en_US
dc.identifierhttps://doi.org/10.13016/M29K45W5Z
dc.identifier.citationScientific Reports (2015) 5:17585 doi: 10.1038/srep17585en_US
dc.identifier.urihttp://hdl.handle.net/1903/20598
dc.language.isoen_USen_US
dc.relation.isAvailableAtCollege of Computer, Mathematical & Physical Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtBiologyen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us
dc.titleQUANTITY: An Isobaric Tag for Quantitative Glycomicsen_US
dc.typeArticleen_US

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