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EXPRESSING DEINOCOCCUS RADIODURANS RECD IN ESCHERICHIA COLI: PHENOTYPIC EFFECTS IN RECBCD(-) AND RECD(-) CELLS

dc.contributor.advisorJulin, Douglas Aen_US
dc.contributor.authorpolansky, steven carlen_US
dc.date.accessioned2007-06-22T05:30:21Z
dc.date.available2007-06-22T05:30:21Z
dc.date.issued2006-12-20
dc.identifier.urihttp://hdl.handle.net/1903/6650
dc.description.abstractThe RecD helicase is a member of the RecBCD complex, which is essential for repair of double-stranded breaks (DSBs) in DNA via homologous recombination in Escherichia coli. The microbe Deinococcus radiodurans is capable of fixing high amounts of DSBs, owing to an efficient repair system. However, Deinococcus does not contain any recB or recC genes; only a RecD-like helicase has been observed. In Escherichia coli strains mutant for the native RecD subunit, the D. radiodurans RecD cannot restore nuclease activity when infected with T4 gene2- bacteriophage. Cell viability tests and mitomycin C exposure of RecBC(+)D(-) strains show no adverse effects from the Dr RecD. A negative phenotype was encountered with strains lacking RecBCD and expressing D. radiodurans RecD protein. Microscopy studies of RecBCD(-) E. coli expressing the D. radiodurans RecD helicase show long cellular structures termed filaments. The Dr RecD protein may be binding to and disrupting the replication fork.en_US
dc.format.extent3410485 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.titleEXPRESSING DEINOCOCCUS RADIODURANS RECD IN ESCHERICHIA COLI: PHENOTYPIC EFFECTS IN RECBCD(-) AND RECD(-) CELLSen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentBiochemistryen_US
dc.subject.pqcontrolledChemistry, Biochemistryen_US
dc.subject.pqcontrolledChemistry, Biochemistryen_US
dc.subject.pquncontrolledfilamentsen_US
dc.subject.pquncontrolledSOSen_US
dc.subject.pquncontrolledreplication forken_US
dc.subject.pquncontrolledRecDen_US
dc.subject.pquncontrolledDeinococcus radioduransen_US
dc.subject.pquncontrolledhelicaseen_US


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