SITE-DIRECTED MUTAGENESIS OF GROEL:DEVELOPING A SYSTEM FOR MONITORING ALLOSTERIC MOVEMENTS BY FLUORESCENCE RESONANCE ENERGY TRANSFER
SITE-DIRECTED MUTAGENESIS OF GROEL:DEVELOPING A SYSTEM FOR MONITORING ALLOSTERIC MOVEMENTS BY FLUORESCENCE RESONANCE ENERGY TRANSFER
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Date
2007-01-02
Authors
Yang, Yu
Advisor
Lorimer, George H
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Abstract
The Escherichia coli chaperonin protein GroEL can assist protein folding to its native state through the consumption of ATP. Accompanying this process, GroEL undergoes structural change, resulting in an expansion of the central cavity. Monitoring apical domain movement by fluorescence resonance energy transfer (FRET) between two mobile apical fluorophores, can provide information about the GroEL allosteric transitions. To reach this goal, the three native cysteine residues on each subunit of wild type GroEL were removed and a new cysteine site in the apical domain was introduced by site-directed mutagenesis. Fluorescent probes were attached to the cysteine residues, allowing us to perform FRET experiments. The observed change of FRET efficiency (E) reported the GroEL structural changes.