SITE-DIRECTED MUTAGENESIS OF GROEL:DEVELOPING A SYSTEM FOR MONITORING ALLOSTERIC MOVEMENTS BY FLUORESCENCE RESONANCE ENERGY TRANSFER

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dc.contributor.advisorLorimer, George Hen_US
dc.contributor.authorYang, Yuen_US
dc.contributor.departmentBiochemistryen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.date.accessioned2007-02-01T20:24:35Z
dc.date.available2007-02-01T20:24:35Z
dc.date.issued2007-01-02en_US
dc.description.abstractThe Escherichia coli chaperonin protein GroEL can assist protein folding to its native state through the consumption of ATP. Accompanying this process, GroEL undergoes structural change, resulting in an expansion of the central cavity. Monitoring apical domain movement by fluorescence resonance energy transfer (FRET) between two mobile apical fluorophores, can provide information about the GroEL allosteric transitions. To reach this goal, the three native cysteine residues on each subunit of wild type GroEL were removed and a new cysteine site in the apical domain was introduced by site-directed mutagenesis. Fluorescent probes were attached to the cysteine residues, allowing us to perform FRET experiments. The observed change of FRET efficiency (E) reported the GroEL structural changes.en_US
dc.format.extent1928617 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/1903/4256
dc.language.isoen_US
dc.subject.pqcontrolledChemistry, Biochemistryen_US
dc.titleSITE-DIRECTED MUTAGENESIS OF GROEL:DEVELOPING A SYSTEM FOR MONITORING ALLOSTERIC MOVEMENTS BY FLUORESCENCE RESONANCE ENERGY TRANSFERen_US
dc.typeThesisen_US

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