Show simple item record

EXPRESSION AND DISTRIBUTION OF BUTYROPHILIN 1A1 AND XANTHINE DEHYDROGENASE-OXIDASE IN LACTATING MAMMARY GLAND

dc.contributor.advisorMather, Ianen_US
dc.contributor.authorTao, Lien_US
dc.date.accessioned2006-09-12T05:32:25Z
dc.date.available2006-09-12T05:32:25Z
dc.date.issued2006-05-12en_US
dc.identifier.urihttp://hdl.handle.net/1903/3698
dc.description.abstractMilk-lipid droplets are secreted from mammary epithelial cells with an outer envelope of membrane, called the milk lipid globule membrane, which consists of a phospholipid bilayer and protein coat. Constituents of this protein coat, including butyrophilin 1a1 (Btn1a1) and xanthine dehydrogenase/ oxidase (Xdh/Xo), are presumed to function in milk-lipid secretion. We postulate that Btn1a1 is targeted to the apical membrane and binds to Xdh/Xo, which is present on the surface of intracellular lipid droplets. To determine the distribution of Btn1a1 and Xdh/Xo in lactating mammary tissue, we introduced recombinant adenoviral vectors encoding Btn1a1 or Xdh/Xo fused to variants of green fluorescent protein (GFP) into mouse mammary gland. The distribution of the respective expressed fusion proteins was determined by confocal microscopy. Results showed that Btn1a1 and Xdh/Xo were targeted to the apical plasma membrane and the surface of intracellular lipid droplets, respectively. Study of the distribution of either fusion proteins in the secreted droplets showed that the membrane forms crescent-like and blebbing structures, thus confirming earlier electron microscopic data, which suggested that the milk-lipid droplet membrane undergoes structural rearrangement after secretion.en_US
dc.format.extent1594577 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen_US
dc.titleEXPRESSION AND DISTRIBUTION OF BUTYROPHILIN 1A1 AND XANTHINE DEHYDROGENASE-OXIDASE IN LACTATING MAMMARY GLANDen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentCell Biology & Molecular Geneticsen_US
dc.subject.pqcontrolledBiology, Cellen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record