The agarase system of saccharophagus degradans strain 2-40 : analysis of the agarase system and protein localization
The agarase system of saccharophagus degradans strain 2-40 : analysis of the agarase system and protein localization
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Date
2005-12-05
Authors
Ekborg, Nathan Alexander
Advisor
Weiner, Ronald
Hutcheson, Steven
Hutcheson, Steven
Citation
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Abstract
Saccharophagus degradans (formerly "Microbulbifer degradans") strain 2-40 is a Gram-negative marine bacterium isolated from the Chesapeake Bay. Analysis of 16s rDNA sequence indicated that S. degradans is related to a group of marine proteobacteria adept at degrading complex polysaccharides (CPs). S. degradans can depolymerize at least ten CPs including agarose. Agarose, an algal galactan, is degraded by few organisms. The agarase system of S. degradans was shown to be composed of five enzymes AgaA, AgaB, AgaC, AgaD and AgaE. These proteins contain glycoside hydrolase domains GH16, GH50 and GH86. S. degradans is the only organism known to collectively encode agarases with at least one of these domains. Unusual for agarases, AgaB and AgaE also contain multiple type-six carbohydrate binding modules. Furthermore, AgaE contains four thrombospondin type-three repeats whose function in prokaryotic proteins were unknown. The predicted agarases were characterized using a variety of methods including genomics, biochemical assays, proteomics and a newly described mutagenic technique. Agar degradation by S. degradans includes two depolymerases, AgaB and AgaC, a B-agarase II (AgaE) and a possible a-neoagarobiose hydrolase (AgaA). AgaB was found to be freely secreted while AgaC and AgaE were surface associated. AgaC is a predicted lipoprotein while AgaE did not have domains characteristic of surface localization. The Tsp-3 repeats, which are similar to repeats found on other cell surface enzymes, are the proposed cell surface anchoring sequences of AgaE.