University of Maryland LibrariesDigital Repository at the University of Maryland
    • Login
    View Item 
    •   DRUM
    • Theses and Dissertations from UMD
    • UMD Theses and Dissertations
    • View Item
    •   DRUM
    • Theses and Dissertations from UMD
    • UMD Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    MEASUREMENT OF IN SITU EXPRESSION OF PROTEORHODOPSIN GENES AT THE NORTH PACIFIC CENTRAL GYRE STATION ALOHA

    Thumbnail
    View/Open
    umi-umd-2778.pdf (5.349Mb)
    No. of downloads: 1213

    Date
    2005-08-10
    Author
    Shi, Yanmei
    Advisor
    Suzuki, Marcelino T
    Metadata
    Show full item record
    Abstract
    A novel approach was developed for the measurement of microbial gene expression in the oceans, and used to test the hypothesis that Proteorhodopsin (PR) gene expression is regulated at the transcriptional level. Chapter 1 describes the optimization of total nucleic-acid extraction that allows "quasi-absolute" quantification of PR mRNAs and coding genes, and the subsequent use of mRNA to gene ratio as a measure of PR gene transcription. In Chapter 2, I describe the construction of a PR cDNA clone library using an RNA sample collected from the North Pacific Central Gyre Station ALOHA, from which diverse PR gene sequences were retrieved and subjected to phylogenetic analyses based on the amino acid sequences. Chapter 3 describes the measurement of in situ PR gene transcription during three diel cycles. Light availability and physical processes such as advection were both found to affect measured PR gene transcription at 25 m at station ALOHA.
    URI
    http://hdl.handle.net/1903/2982
    Collections
    • Biology Theses and Dissertations
    • MEES Theses and Dissertations
    • UMD Theses and Dissertations

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility
     

     

    Browse

    All of DRUMCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister
    Pages
    About DRUMAbout Download Statistics

    DRUM is brought to you by the University of Maryland Libraries
    University of Maryland, College Park, MD 20742-7011 (301)314-1328.
    Please send us your comments.
    Web Accessibility