Protein-based vehicles for biomimetic RNAi delivery
Protein-based vehicles for biomimetic RNAi delivery
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Date
2019-02-26
Authors
Pottash, Alex Eli
Kuffner, Christopher
Noonan-Shueh, Madeleine
Jay, Steven M.
Advisor
Citation
Pottash, A.E., Kuffner, C., Noonan-Shueh, M. et al. Protein-based vehicles for biomimetic RNAi delivery. J Biol Eng 13, 19 (2019) doi:10.1186/s13036-018-0130-7
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Abstract
Broad translational success of RNA interference (RNAi) technology depends on the development of effective
delivery approaches. To that end, researchers have developed a variety of strategies, including chemical
modification of RNA, viral and non-viral transfection approaches, and incorporation with delivery vehicles such
as polymer- and lipid-based nanoparticles, engineered and native proteins, extracellular vesicles (EVs), and others.
Among these, EVs and protein-based vehicles stand out as biomimetically-inspired approaches, as both proteins
(e.g. Apolipoprotein A-1, Argonaute 2, and Arc) and EVs mediate intercellular RNA transfer physiologically. Proteins
specifically offer significant therapeutic potential due to their biophysical and biochemical properties as well as their
ability to facilitate and tolerate manipulation; these characteristics have made proteins highly successful translational
therapeutic molecules in the last two decades. This review covers engineered protein vehicles for RNAi delivery
along with what is currently known about naturally-occurring extracellular RNA carriers towards uncovering design
rules that will inform future engineering of protein-based vehicles.
Notes
Partial funding for Open Access provided by the UMD Libraries' Open Access Publishing Fund.