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dc.contributor.advisorHill, Russell Ten_US
dc.contributor.authorMajor, Samuelen_US
dc.date.accessioned2018-10-12T05:30:23Z
dc.date.available2018-10-12T05:30:23Z
dc.date.issued2018en_US
dc.identifierhttps://doi.org/10.13016/M2KH0F319
dc.identifier.urihttp://hdl.handle.net/1903/21453
dc.description.abstractPonds in Frederick, MD were fertilized with chicken manure to increase the nutrient load in the water and stimulate microalgal growth. Nutrient analyses indicate that fertilization results in significant increases in the DOC, TDN, and TDP. The bacterial and eukaryotic microalgal communities were analyzed using 16S and 18S rRNA gene sequencing, respectively. Communities were analyzed pre-fertilization and for 15 days following fertilization. Molecular data reveals a decrease in diversity as microalgal blooms form. The microalgal density increased following fertilization, with enrichment for the Chlamydomonadales order. Prior to fertilization the bacterial communities were dominated by five phyla: Actinobacteria, Bacteroidetes, Cyanobacteria, Proteobacteria, and Verrucomicrobia. Dominant bacterial genera post-fertilization included Flavobacterium, Limnohabitans, and Polynucleobacter. Bacteria isolated from the ponds were screened for effects on Scenedesmus sp. HTB1 to identify bacteria that either enhance or inhibit microalgal growth. The growth-promoting bacteria were closely related to bacteria found to be enriched during microalgal bloom formation.en_US
dc.language.isoenen_US
dc.titleTHE PROBIOTICS OF BIOFUEL: A METAGENOMIC STUDY OF MICROALGAE GROWN FOR FUEL PRODUCTIONen_US
dc.typeThesisen_US
dc.contributor.publisherDigital Repository at the University of Marylanden_US
dc.contributor.publisherUniversity of Maryland (College Park, Md.)en_US
dc.contributor.departmentMarine-Estuarine-Environmental Sciencesen_US
dc.subject.pqcontrolledMicrobiologyen_US
dc.subject.pqcontrolledEcologyen_US
dc.subject.pqcontrolledMolecular biologyen_US
dc.subject.pquncontrolledBacteriaen_US
dc.subject.pquncontrolledBiofuelsen_US
dc.subject.pquncontrolledEcologyen_US
dc.subject.pquncontrolledMetagenomicsen_US
dc.subject.pquncontrolledMicroalgaeen_US
dc.subject.pquncontrolledProbioticsen_US


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