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Comparative analysis of a sex chromosome from the blackchin tilapia, Sarotherodon melanotheron

dc.contributor.authorGammerdinger, William J.
dc.contributor.authorConte, Matthew A.
dc.contributor.authorBaroiller, Jean-François
dc.contributor.authorD’Cotta, Helena
dc.contributor.authorKocher, Thomas D.
dc.date.accessioned2017-08-31T19:52:28Z
dc.date.available2017-08-31T19:52:28Z
dc.date.issued2016
dc.identifierhttps://doi.org/10.13016/M2BR8MG60
dc.identifier.citationGammerdinger, W.J., Conte, M.A., Baroiller, JF. et al. Comparative analysis of a sex chromosome from the blackchin tilapia, Sarotherodon melanotheron . BMC Genomics 17, 808 (2016).en_US
dc.identifier.urihttp://hdl.handle.net/1903/19703
dc.descriptionPartial funding for Open Access provided by the UMD Libraries' Open Access Publishing Fund.en_US
dc.description.abstractBackground: Inversions and other structural polymorphisms often reduce the rate of recombination between sex chromosomes, making it impossible to fine map sex-determination loci using traditional genetic mapping techniques. Here we compare distantly related species of tilapia that each segregate an XY system of sex-determination on linkage group 1. We use whole genome sequencing to identify shared sex-patterned polymorphisms, which are candidates for the ancestral sex-determination mutation. Results: We found that Sarotherodon melanotheron segregates an XY system on LG1 in the same region identified in Oreochromis niloticus. Both species have higher densities of sex-patterned SNPs, as well as elevated number of ancestral copy number variants in this region when compared to the rest of the genome, but the pattern of differentiation along LG1 differs between species. The number of sex-patterned SNPs shared by the two species is small, but larger than expected by chance, suggesting that a novel Y-chromosome arose just before the divergence of the two species. We identified a shared sex-patterned SNP that alters a Gata4 binding site near Wilms tumor protein that might be responsible for sex-determination. Conclusions: Shared sex-patterned SNPs, insertions and deletions suggest an ancestral sex-determination system that is common to both S. melanotheron and O. niloticus. Functional analyses are needed to evaluate shared SNPs near candidate genes that might play a role in sex-determination of these species. Interspecific variation in the sex chromosomes of tilapia species provides an excellent model system for understanding the evolution of vertebrate sex chromosomes.en_US
dc.description.urihttps://doi.org/10.1186/s12864-016-3163-7
dc.language.isoen_USen_US
dc.publisherBioMed Centralen_US
dc.subjectAfrican cichlidsen_US
dc.subjectSex chromosome evolutionen_US
dc.subjectSex-determinationen_US
dc.titleComparative analysis of a sex chromosome from the blackchin tilapia, Sarotherodon melanotheronen_US
dc.typeArticleen_US
dc.relation.isAvailableAtCollege of Computer, Mathematical & Physical Sciencesen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtBiologyen_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us


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