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dc.contributor.authorChakrabarti, Kristi R.
dc.contributor.authorAndorko, James I.
dc.contributor.authorWhipple, Rebecca A.
dc.contributor.authorZhang, Peipei
dc.contributor.authorSooklal, Elisabeth L.
dc.contributor.authorMartin, Stuart S.
dc.contributor.authorJewell, Christopher M.
dc.date.accessioned2017-08-31T16:39:17Z
dc.date.available2017-08-31T16:39:17Z
dc.date.issued2016-02-08
dc.identifierhttps://doi.org/10.13016/M21G0HV3N
dc.identifier.citationOncotarget. 2016; 7:10486-10497. https://doi.org/10.18632/oncotarget.7251en_US
dc.identifier.urihttp://hdl.handle.net/1903/19690
dc.descriptionFunding for Open Access provided by the UMD Libraries' Open Access Publishing Fund.en_US
dc.description.abstractFree-floating tumor cells located in the blood of cancer patients, known as circulating tumor cells (CTCs), have become key targets for studying metastasis. However, effective strategies to study the free-floating behavior of tumor cells in vitro have been a major barrier limiting the understanding of the functional properties of CTCs. Upon extracellular-matrix (ECM) detachment, breast tumor cells form tubulin-based protrusions known as microtentacles (McTNs) that play a role in the aggregation and re-attachment of tumor cells to increase their metastatic efficiency. In this study, we have designed a strategy to spatially immobilize ECM-detached tumor cells while maintaining their free-floating character. We use polyelectrolyte multilayers deposited on microfluidic substrates to prevent tumor cell adhesion and the addition of lipid moieties to tether tumor cells to these surfaces through interactions with the cell membranes. This coating remains optically clear, allowing capture of high-resolution images and videos of McTNs on viable free-floating cells. In addition, we show that tethering allows for the real-time analysis of McTN dynamics on individual tumor cells and in response to tubulin-targeting drugs. The ability to image detached tumor cells can vastly enhance our understanding of CTCs under conditions that better recapitulate the microenvironments they encounter during metastasis.en_US
dc.language.isoen_USen_US
dc.publisherImpact Journalsen_US
dc.subjectcirculating tumor cellsen_US
dc.subjectmicrotentaclesen_US
dc.subjectbreast canceren_US
dc.subjectmicrofluidicsen_US
dc.subjectpolyelectrolyte multilayersen_US
dc.titleLipid tethering of breast tumor cells enables real-time imaging of free-floating cell dynamics and drug responseen_US
dc.typeArticleen_US
dc.relation.isAvailableAtA. James Clark School of Engineeringen_us
dc.relation.isAvailableAtFischell Department of Bioengineeringen_us
dc.relation.isAvailableAtDigital Repository at the University of Marylanden_us
dc.relation.isAvailableAtUniversity of Maryland (College Park, MD)en_us


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